Amytracker 520 is our green optotracer for labeling protein aggregates with repetitive arrangement of β-sheets. It labels Aβ plaques and neurofibrillary tangles in tissue sections with AD pathology and α-synuclein aggregates in tissue sections with PD pathology. Contact us to learn more about Amytracker applications. As all our optotracers, Amytracker 520 is exceptionally photostable and fluorogenic. When bound to a target, Amytracker 520 can be imaged using epifluorescence, confocal and superresolution microscopy. Spectral information can be acquired using a fluorescence spectrophotometer. Use recommended filter sets as well as excitation- and emission wavelengths according to the following table. Table: Excitation- and emission wavelengths as well as recommended filter sets. Exmax Emmax Recommended filter-sets Amytracker 520 460 nm 520 nm FITC, GFP Amytracker 520 is available in four different formulations (See volumes and prices in the drop-down list below): Aqueous: 1 mg/ml solution in ultrapure water. The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. To prevent evaporation of the aqueous solvent, close the container carefully after use, spin down liquid and use up small volumes quickly. DMSO: 1 mg/ml solution in DMSO to prevent solvent evaporation. The product should be diluted 1:1000 before use. For use in live-cells, sometimes 1:500 is necessary due to uptake limitations. Solid: 1 mg solid lyophilised in a sterile injection bottle. We recommend dilution to 4 mg/ml in physiological saline followed by intravenous injection with a total dose of 5 mg/KG. Drop&Shine: 5 ml ready-to-use product in mounting medium. Ideal for use in tissue sections. Add a some Drop&Shine and mount your slide to detect amyloids within minutes. Documents ▽ Product Information MSDS (Aqueous) MSDS (DMSO) MSDS (Solid) MSDS (Drop&Shine)
