Amorphous nickel-titanium perforated foils (nitinol) on gold or copper mesh for cryo-EM. They are characterized by defined hole sizes (round, size/spacing), excellent conductivity, and user-friendly handling. The advantages include improved resolution, faster sample preparation, and higher data acquisition efficiency. The films are on the matte side (grey/black), the reverse side is metallic and shiny (gold or copper). To hydrophilize the metallic perforated films, we recommend the Pelco easiGlow (15mA, 30-60s). Sample concentration using apoferritin as an example: 1 mg/ml to 1.5 mg/ml. 🔹 Technical specifications • Material: Amorphous Nickel Titanium (NiTi/Nitinol)• Carrier material: Gold or Copper mesh• Hole pattern: Round holes with defined diameter and spacing• Surface: o Matte side (grey/black) = active film side o Back side metallic shiny (gold or copper colored)• Hydrophilization: Recommendation Pelco easiGlow (15 mA, 30–60 s)• Example sample concentration: Apoferritin 1.0–1.5 mg/ml • Packaging unit: 5 pieces 🔹 Main advantages 1. Improved resolution of the reconstruction o Minimized electron charging & reduced beam-induced motion (BIM).2. Faster sample preparation o Lower adsorption capacity, more uniform particle distribution.3. More efficient data collection o Higher particle density per hole, fewer micrograms required.4. Easy integration o Compatible with all common cryo-EM workflows. ANTcryo ANTA Cryo-EM Grids - Datasheet (English) Download "ANTcryo ANTA Cryo-EM Grids - Datasheet (English)" (814.73 KB)
Related