APC1 (E684) Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS1611-50, BS1611-100 Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q9H1A4 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB IHC IF All Applications: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 Background: APC1 is 1 of at least 11 subunits of the anaphase promoting complex (APC), which functions at the metaphase to anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: APC1 (E684) polyclonal antibody detects endogenous levels of APC1 protein. Immunogen: Synthetic peptide, corresponding to amino acids 650-698 of Human APC1. Conjugate: Unconjugated Modification: Unmodified Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot (WB) analysis of APC1 (E684) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:BV2 whole cell lysate(40ug) Lane4:HCT116 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug), Immunohistochemistry (IHC) analyzes of APC1 (E684) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasm and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
