Calcium is one of the most important elements in a reef aquarium. Reef invertebrates, such as corals, crustaceans, mollusks, and even some type of encrusting algae, need calcium to grow and remain healthy. Over time, the calcium level drops as it is used by invertebrates, or as it reacts with excess phosphate. The API CALCIUM TEST KIT measures calcium levels as low as 20 mg/L to help you keep your reef inhabitants healthy. Whats in the Box 2 Test Bottles; Easy-To-Ready Instructions; Glass Test Tubes Use Instructions To remove childproof safety cap: With one hand, push red tab left with thumb while unscrewing cap with free hand. 1. Rinse a clean test tube with the water being tested. 2. Fill the test tube with 5 ml of water to be tested (to the line on the tube). 3. Holding the bottle vertically, add 10 drops of Calcium Test Solution Bottle #1, cap the test tube and shake for 10 seconds. 4. Shake Calcium Test Solution Bottle #2 vigorously for 10 seconds. 5. Holding the bottle vertically, add Calcium Test Solution Bottle #2, drop by drop to the test tube until the solution changes from pink to the blue endpoint. Be sure to count the number of drops added. Cap and shake the test tube between drops. 6. As the endpoint is approached, the color will usually change from pink to purple before finally changing to blue. After the purple color forms, only 1-2 more drops should be required to reach the blue endpoint. Use the chart on the back of this insert to determine the calcium level. Storage Instructions Store in a cool, dry, well-ventilated area. Keep container tightly closed Caution Keep locked up and out of the reach of children