Bacterial Artificial Chromosomes, Second Editionexpands upon the previous edition with current, detailed methods developed for working with BACs. Updated chapters included in this edition present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model organisms, and medical genetics and drug discovery. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and tips to troubleshoot and avoid known pitfalls.Authoritative and cutting-edge,Bacterial Artificial Chromosomes, Second Editionseeks to aid scientists in advancing their research using these exciting BAC techniques and strategies.
