Description Bacterial DNA Isolation kit provides a quick and easy method of extracting genomic DNA from cultures of bacteria (Gram-negative and Gram-positive) from various sources; it can process less than 3ml of bacterial cultures in the logarithmic growth phase (1×109 bacteria). The kit is matched with high-efficiency Foregene Protease, which allows 15-50μg of high-quality genomic DNA to be extracted within 1 hour. In addition, the kit can also extract genetic material other than the genome, such as plasmids, cosmid, BAC, etc. The DNA-only silica gel matrix material used in the spin column is a unique new material of the company, which can efficiently and specifically adsorb DNA. The maximum adsorption capacity for DNA is 80μg. The unique buffer and elution system can maximize the removal of RNA, impurity proteins, ions and other organic compounds in cells. The extracted genomic DNA fragment is large, high purity, stable and reliable quality, and the size of the DNA fragment is stable at about 23kb. Kit components Kit contents DE-05311 50 T Buffer ML1 25ml Buffer ML2 25ml Buffer PW 30ml Buffer WB 25ml Buffer EB 10ml Foregene Protease 1.25ml Lysozyme 250mg Buffer TE 5ml DNA-Only Column 50 sets IFU 1 piece Specifications Model Spin-column type Purification components Foregene Spin column, reagent Flux 1-24 samples Preparation time ~60min(24 samples) Centrifuge Table centrifuge Bacterial lysate separation centrifugal separation Liquid volume of spin column 800μl Purification column DNA load carrying capacity 80μg Elution volume 100-200μl Bacteria processing volume ≤3ml (Cultivate bacteria for 16-20hr) Features No RNase contamination: No need to add RNase to remove the RNA in the genomic DNA, avoiding the laboratory from being contaminated by exogenous RNase. Fast speed: Foregene Protease has higher activity than similar proteases and can digest broken bacterial samples in a short time. Convenience: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA. Safety: no organic reagent extraction is required. High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments. Application Bacterial DNA Isolation kit is suitable for purifying genomic DNA of the following samples: Gram-negative bacteria and Gram-positive bacteria in the logarithmic growth phase. Work Flow Storage and Shelf life This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C. Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to put the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use. Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months); when stored at 4°C, its activity and stability will be better, so it is recommended to store it at 4°C, remember not to keep it at -20°C. Lysozyme is a dry powder, which is active when stored at 2-8°C for a long time (3 months). For longer storage, please store it at -20°C. Documents Bacterial DNA Isolation Kit Instruction Manual-V3.0-2203-EN
