Bacterial Sortase Substrate I Catalog number: B2011394 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 1 mg Molecular Weight or Concentration: 1015.2 g/mol Supplied as: Lyophilized Applications: molecular tool for various biochemical applications Storage: -20 °C Keywords: Bacterial Sortase Substrate I, FRET Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Piper IM, Struyvenberg SA, Valgardson JD, Johnson DA, Gao M, Johnston K, Svendsen JE, Kodama HM, Hvorecny KL, Antos JM, Amacher JF. Sequence variation in the β7-β8 loop of bacterial class A sortase enzymes alters substrate selectivity J Biol Chem. 2021 Aug;297(2):100981. Chen I, Dorr BM, Liu DR. A general strategy for the evolution of bond-forming enzymes using yeast display Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404. Bhat AH, Nguyen MT, Das A, Ton-That H. Anchoring surface proteins to the bacterial cell wall by sortase enzymes: how it started and what we know now Curr Opin Microbiol. 2021 Apr;60:73-79. Hansenová Maňásková S, Bikker FJ, Nazmi K, van Zuidam R, Slotman JA, van Cappellen WA, Houtsmuller AB, Veerman EC, Kaman WE. Incorporation of a Valine-Leucine-Lysine-Containing Substrate in the Bacterial Cell Wall Bioconjug Chem. 2016 Oct 19;27(10):2418-2423. Yin JC, Fei CH, Lo YC, Hsiao YY, Chang JC, Nix JC, Chang YY, Yang LW, Huang IH, Wang S. Structural Insights into Substrate Recognition by Clostridium difficile Sortase Front Cell Infect Microbiol. 2016 Nov 22;6:160. Clancy KW, Melvin JA, McCafferty DG. Sortase transpeptidases: insights into mechanism, substrate specificity, and inhibition Biopolymers. 2010;94(4):385-96. Zuo C, Ding R, Wu X, Wang Y, Chu GC, Liang LJ, Ai H, Tong ZB, Mao J, Zheng Q, Wang T, Li Z, Liu L, Sun D. Thioester-Assisted Sortase-A-Mediated Ligation Angew Chem Int Ed Engl. 2022 Jul 11;61(28):e202201887. Podracky CJ, An C, DeSousa A, Dorr BM, Walsh DM, Liu DR. Laboratory evolution of a sortase enzyme that modifies amyloid-β protein Nat Chem Biol. 2021 Mar;17(3):317-325. Jacobitz AW, Kattke MD, Wereszczynski J, Clubb RT. Sortase Transpeptidases: Structural Biology and Catalytic Mechanism Adv Protein Chem Struct Biol. 2017;109:223-264.
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