BES Buffering Agent Catalog number: B2015435 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 10 g Molecular Weight or Concentration: 213.25 g/mol Supplied as: crystalline powder Applications: a molecular tool for various biochemical applications Storage: RT Keywords: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid; N,N-Bis(2-hydroxyethyl)taurine Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Olson DW, Fujimoto Y, Madsen KL, Stewart BG, Carle M, Zeng J, Jewell L, Sheasgreen JL, Chong FT, Kneteman NM, Bigam DL, Churchill TA. Potentiating the benefit of vascular-supplied glutamine during small bowel storage: importance of buffering agent Transplantation. 2002 Jan 27;73(2):178-85. Pulis RP, Wu BM, Kneteman NM, Churchill TA. Conservation of phosphorylation state of cardiac phosphofructokinase during in vitro hypothermic hypoxia Am J Physiol Heart Circ Physiol. 2000 Nov;279(5):H2151-8. MacKerrow SD, Merry JM, Hoeprich PD. Effect of buffers on testing of Candida species susceptibility to flucytosine J Clin Microbiol. 1987 May;25(5):885-8. Mandolino C, Pizarro MD, Quintana AB, Rodríguez JV, Mamprin ME. Hypothermic preservation of rat liver microorgans (LMOs) in bes-gluconate solution. Protective effects of polyethyleneglycol (PEG) on total water content and functional viability Ann Hepatol. 2011 Apr-Jun;10(2):196-206. Fujimoto Y, Olson DW, Madsen KL, Zeng J, Jewell LD, Kneteman NM, Bigam DL, Churchill TA. Defining the role of a tailored luminal solution for small bowel preservation Am J Transplant. 2002 Mar;2(3):229-36. Fujita Y, Tokunaga T, Kataoka H. Saline and buffers minimize the action of interfering factors in the bacterial endotoxins test Anal Biochem. 2011 Feb 1;409(1):46-53. Mamprin ME, Petrocelli S, Guibert E, Rodriguez J. A novel BES-gluconate-sucrose (BGS) solution for cold storage of isolated hepatocytes Cryo Letters. 2008 Mar-Apr;29(2):121-33. Vogel H, Gerlach C, Richert C. Reactions of buffers in cyanogen bromide-induced ligations Nucleosides Nucleotides Nucleic Acids. 2013;32(1):17-27. Gäb J, John H, Melzer M, Blum MM. Stable adducts of nerve agents sarin, soman and cyclosarin with TRIS, TES and related buffer compounds–characterization by LC-ESI-MS/MS and NMR and implications for analytical chemistry J Chromatogr B Analyt Technol Biomed Life Sci. 2010 May 15;878(17-18):1382-90.
