Bioneer CycleScript Reverse Transcriptase (10,000 Units)

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Bioneer CycleScript™ Reverse Transcriptase (10,000 Units) Buy Bioneer products from MSE Supplies at the best value. CycleScript™ Reverse Transcriptase uses a stabilizer to synthesi ze cDNA even at high temperature (55°C). Not only this product is capable of performing traditional fixed temperature reverse transcription (FTRT), but also cyclic reverse transcription(CRT) through sequential temperature change in 2-3 steps. ※This product is shipped in dry ice. Features and Benefits Flexible Reaction Conditions Cyclic temperature reverse transcription reaction performs primer annealing at low temperature (15 to 40°C). As the secondary structure of template RNA is released through repeated application of high temperature (50 to 55°C), cDNA can be synthesized with more efficiency than the conventional 42°C reverse reaction. Furthermore, reverse transcription is also possible at a fixed temperature (22 ~ 55°C). Stability Higher thermostability than conventional reverse transcriptase capable of undergoing the reverse transcription up to 55 ℃. Easy of Use Shortened reaction time not requiring pre-incubation process of primer and RNA template during the performance of cyclic reverse transcription, with simplified procedure and reduced reaction time Reproducibility Reproducible results with uniform quality products for each batch under the ISO 9001 quality system Applications First-strand synthesis of cDNA from RNA molecules RT-PCR Random priming reaction Library construction Probe labeling mRNA 5' end mapping by primer extension analysis. Specifications 5' to 3' exonuclease activity No 3' to 5' exonuclease activity No 3' – A overhang No Strand displacement Yes Fragment size Up to 9 kb Components 5x Reaction Buffer: Tris-HCl, KCl, MgCl2 (pH8.1) 100 mM DTT 10 mM dNTPs mix: 2.5 mM of each dNTP Concentration 10,000 Unit (200 U/μl) Storage Conditions 50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50 mM (NH4)2SO4, Stabilizers. Storage Temperature -20°C Unit Definition One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly (A) oligo (dT) as template primer. Show more