Bioneer RocketScript Reverse Transcriptase (50,000 Units)

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Bioneer RocketScript™ Reverse Transcriptase (50,000 Units) Buy Bioneer products from MSE Supplies at the best value. RocketScriptTM Reverse Transcriptase is an independently develo ped thermostable reverse transcriptase derived from M-MLV. Its resistance to heat (42 ~ 70℃) allows effective synthesis of complex RNA structures with full-length cDNA. ※This product is shipped in dry ice. Features and Benefits Thermostability Performance at comprehensive temperature conditions from 42 to 70 ℃ for cDNA synthesis with Rocketscript ™ series overcoming the limitation of traditional 42°C RT reactions User-friendly Simplified procedure by including all components necessary for cDNA synthesis such as Thermostable Reverse Transcriptase, RNase inhibitor, etc. Reproducibility Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system Applications Gene synthesis First-strand synthesis of cDNA from RNA molecules (reverse transcription) RT-PCR Random priming reactions Library construction Probe labeling mRNA 5'-end mapping by primer extension analysis Real time PCR Specifications 5' to 3' exonuclease No 3' to 5' exonuclease No 3' – A overhang No Fragment size Up to 10 kb Components 5x Reaction Buffer: Tris-HCl, KCl, MgCl2, Stabilizers (pH8.5) 100 mM DTT 10 mM dNTPs mix: 2.5 mM of each dNTP Concentration 10,000 Units (200 U/μl) Storage Conditions 50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, Stabilizers. Storage Temperature -20°C Show more