Product Specifications: Item# 1003-B: Biotin p24 HIV-1 IIIB Mass/Vial: 50µg Vol./Vial: 50ul,50ug protein Diluent: 50mM Dodium Phosphate, 10mM NaC1,pH7.5 Stabilizer: BSA Preservative: 0.02% Storage: 4°C Stability: 12 months at 4°C Minimum Dilution: 1 : 1000 Application: T-Cell Research, Diagnostics, Drug Screening, ELISA, Western ELISA Description: Biotin conjugated to recombinant (baculovirus) HIV-1p24. Coupling Ratio: Approximately 10-20 moles of biotin per mole p24. Specificity: Biotin p24 binds to anti-p24 monoclonal and polyclonal antibodies in Elisa and western Elisa as determined by strepavidin-AP/HRP assays. Biological Activity: Biotin p24 binds to solid phase anti-p24 pAb (oligoclonal) as determined by capture ELISA using strepavidin-HRP as a secondary reagent. Application and Instructions for use Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA may be performed in 1 : 5000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. p24/Anti-p24 pAb capture ELISA may be performed in 1-10ng pAb in solid phase and 0.1-1ng of biotin-p24.
