Description Blue Dextran 2000 (CAS 87915-38-6) is a high-molecular weight polymer (average MW ~2,000,000 Da) covalently conjugated with Cibacron Blue dye, widely used as a premium void volume (V0) marker and molecular weight standard in size-exclusion chromatography (SEC). Specifications Property Specification Product Name Blue Dextran 2000 CAS Number 87915-38-6 Average Molecular Weight ~2,000,000 Da Appearance Blue to dark blue powder or crystalline solid Substitution Degree ~0.06 mmol/g dye Loss on Drying ≤ 3.0% Storage Temperature Ambient temperature (15°C – 30°C), keep tightly sealed and protected from light Shipping Condition Ambient Features Ideal Void Volume Marker: The massive molecular weight (~2,000,000 Da) ensures complete exclusion from most gel filtration matrix pores. High Visual Contrast: Covalently bound to a triazinyl dye (Cibacron Blue F3GA) for easy visual tracking and precise UV/Vis detection (wavelength maximum around 620 nm). Excellent Water Solubility: Dissolves quickly and completely in standard aqueous buffers without requiring any organic solvents. Biofargo Quality Assured: Strict batch-to-batch consistency with low loss on drying (≤ 3%) to ensure reproducible chromatographic runs. Reliable Supply Chain: Available in multiple packing sizes, stocked and shipped directly from our warehouse to keep your lab running smoothly. Applications Size-Exclusion & Gel Filtration Chromatography (SEC): Accurately determine the void volume (V0) of columns, evaluate packing efficiency, and check bed consistency. Column Calibration & QC: Serves as a reliable high-molecular-weight reference standard to calibrate chromatography systems across different runs. Permeability & Barrier Integrity Assays: Ideal for investigating macromolecular transport, diffusion, and epithelial/endothelial monolayer permeability due to its large size. Protein Affinity Studies: Utilizes the Cibacron Blue dye moiety to bind specific proteins, allowing fast separation via void-volume elution. How to Use Preparation: Dissolve Blue Dextran 2000 in your running buffer to a final typical concentration of 1–2 mg/mL. Ensure it is fully dissolved. Sample Clarification: Filter the solution through a 0.22 micron or 0.45 micron membrane filter before injection to protect your column from particulates. Sample Loading: Apply an injection volume equivalent to 1% to 3% of the total column bed volume (Vt) for optimal peak sharpness. Detection: Monitor the elution visually or via a UV-Vis detector set between 600 nm and 640 nm (optimal wavelength maximum around 620 nm). Safety Research Use Only: This product is intended solely for laboratory research and development. Not for human, drug, or veterinary use. Always wear appropriate Personal Protective Equipment (PPE) including safety glasses, lab coats, and gloves during handling. Avoid dust inhalation and direct skin contact. For complete hazard, handling, and disposal instructions, please refer to the Biofargo Safety Data Sheet (SDS). Documents COA SDS FAQ Q1: Why is my Blue Dextran 2000 peak tailing or broader than expected? A: This can occur if the sample is too concentrated or viscous. Try reducing the concentration to 1 mg/mL or decreasing the injection volume. Ensure the column is properly packed. Q2: How do I prepare a stock solution and store it? A: Reconstitute in your preferred aqueous chromatography buffer. Prepared solutions can be stored at 4°C for short-term use, but sterile filtration or adding a preservative (like sodium azide) is recommended to prevent microbial growth. Q3: Can Blue Dextran 2000 be used in any chromatography column? A: It is ideal for size-exclusion chromatography matrixes with exclusion limits below 2,000,000 Da. For columns with extremely large pore sizes (e.g., some ultra-large pore acrylic beads), it may partially enter the matrix. Q4: Is Blue Dextran 2000 stable at room temperature? A: Yes, the powder form is highly stable at ambient temperature when stored in a dark, dry environment. Always keep the container tightly closed. Q5: How do I request bulk quantities or custom packaging? A: Biofargo provides bulk scale packaging for industrial and pilot studies. Please click our "Request Bulk Quote" button or contact our sales team directly for tiered pricing.
