BMEM Human Primed Pluripotent, comprising of BMEM Basal Medium and Primed Supplement, is a highly optimized, chemically defined, animal origin-free (AOF) basal medium and supplement combination for the maintenance and expansion of human induced pluripotent stem cells (hiPSCs) in adherent culture. BMEM Basal Medium is based on the rigorously optimized published formulation1. Primed Supplement is new supplement specifically optimized for compatibility with adaptation of hiPSCs from legacy media such as mTeSR1™, a common problem with chemically defined media. Primed Supplement is optimized for component stability and to allow room temperature shipment. Culture of hiPSCs in BMEM Human Primed Pluripotent results in increased cell proliferation and upregulation of core primed pluripotency factors when compared to legacy formulae1. BMEM Human Primed Pluripotent is formulated for compatibility with weekend-free and no-change feeding schedules protocols. BMEM Human Primed Pluripotent is compatible with a variety of culture matrices, including Corning® Matrigel® GFR, Gibco™ Geltrex™ GFR, R&D Systems™ Cultrex™ UltiMatrix GFR, SigmaAldrich® ECMatrix™-511 E8 Laminin, Gibco™ Recombinant Vitronectin, Corning® Synthemax II-SC. BMEM Human Primed Pluripotent is compatible with a variety of passaging methods including BMEM Dissociation (1002), Gibco™ TrypLE™, Stemcell Technologies™ ReLeSR™, and Gibco™ Versene. Each lot of BMEM Basal Medium and Primed Supplement is performance tested on hiPSC and tested for sterility. Larger unit sizes are available on request.Contains HEPES.
