BMEM Pluripotent Kit, comprising of BMEM Basal Medium and Primed Supplement, is a highly optimized, chemically defined, animal origin-free (AOF) kit for the culture of human induced pluripotent stem cells (hiPSCs). BMEM Basal Medium is based on the rigorously optimized published formulation1. Primed Supplement is new supplement specifically optimized for compatibility with adaptation of hiPSCs from legacy media such as mTeSR1. Primed Supplement is optimized for growth factor stability and to allow room temperature shipment. Increased cell proliferation and upregulation of core primed pluripotency factors1. Supports longterm maintenance of stable karyotype. Compatible with weekend-free feeding schedules. Maintains pH throughout weekend. Compatible with a variety of culture matrices, including Matrigel, Geltrex, Cultrex, Mogengel, laminin 511, vitronectin, and Synthemax. Compatible with a variety of passaging methods including BMEM Dissociation, TrypLE, ReLeSR, Versene, and EDTA. Supports tri-lineage differentiation to cardiomyocytes, neural progenitors, and hepatocytes. Each lot of BMEM Basal Medium and Primed Supplement is performance tested on hiPSC and tested for sterility. Larger unit sizes are available on request.
