Description This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form. Feature Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence Restriction Enzyme Cut Site: GCTCTTC (1/4) Digestion of DNA to obtain specific sticky ends Isolated from a recombinant source Tested for the absence of endonucleases, exonucleases, RNases Application Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G); Linearize plasmid template before in vitro transcription Restriction digest Specification Expression Host Recombinant E. coli with BspQI gene Reaction Temperature 50℃ Storage Buffer 20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol Unit Definition 1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system Components Components No. Name 10664ES76 (500 U) 10664ES86 (2,500 U) 10664ES92 (10 KU) 10664ES98 (100 KU) 10664 BspQI GMP-grade (10 U/μL) 50 μL 250 μL 1 mL 10 mL Shipping and Storage The product is shipped with blue ice and can be stored at -15℃ ~ -25℃ for one year. Documents: Safety Data Sheet MSDS-10664-BspQI GMP-grade (10 U_μL)-HB220701.pdf Manuals 10664_Manual_HB221110_EN.pdf
