The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity. The Viewing Head The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces. OpticsThe infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness. The Stage The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. Transmitted Illumination The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction. Phase Contrast The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light. Epi-fluorescence Illumination For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available. Fluorescence Filters Filter Set Excitation Filter Emission Filter B 450-490nm 520nm G 510-560nm 590nm Mercury HBO Excitation Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths. Band Peak Wavelength UV 334nm, 365nm V 405nm B 436nm G 546nm Y 578nm Digital ImagingThe ultra-sensitive 1.4MP color CCD digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features. Specifications Optical System Infinity-corrected Head Trinocular, 45° incline Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm Ocular Diameter 30mm Ocular Diopters right ocular Eyepieces 10X Photo-port height-adjustable 23mm tube Objective Lenses Infinity-corrected Objective Turret Quadruple Focusing System Focus Adjustment Coaxial Coarse/Fine, adjustable tension Fine Division 0.002mm Stage fixed-height, mechanical X-Y translation Stage Dimensions 172mm x 242mm Stage X-Y Travel Range 75mm x 40mm Specimen Holders Terasaki plate, Petri dish Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable Transmitted Illumination 12V 30W halogenvariable intensity Condenser NA 0.3, iris diaphragm, filter slot Power 110VAC 60Hz Objective Lenses Magnification Corrections NA Working Distance Cover-glass Thickness 4X plan fluor 0.15 28mm 1.5mm 10X plan fluor, phase-contrast 0.30 3mm 1.5mm 20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm 40X plan fluor 0.60 2.23mm 1.5mm Camera Specifications Sensor Type Interline CCD (color) Sensor Optical Format 2/3" Active Pixels 1.4M (1360 x 1024) Pixel Size 6.45µm x 6.45µm Active Sensor Area 8.77mm x 6.6mm Shutter global shutter Sensitivity 1240mV @ 1/30s Spectral Response 380-650nm with IR-cut filter Capture Resolution and Maximum Framerate 15fps @ 1360x1024 Connectivity USB 3.0 Lens-mount C-mount Compensating Lens 0.67X/0.75X Software OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+ Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM Features Windows Version Live video preview Captures still images and video Time-lapse capture Exposure and white-balance controls Dark-field correction Digital binning Image processing Measurement tools, including lines, arcs, polygons Segmentation and counting tools Image-stitching, extended depth-of-field, HDR Fluorescence color-mapping Mac/Linux Versions Live video preview Captures still images and video Exposure control Color adjustment Measurement tools, including lines, arcs, polygons Packing List:One microscope body Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X One pair 10X eyepieces One phase-contrast annulus board One top-mount illuminator One rear-mount fluorescence illuminator One power supply for fluorescence illuminator One UV blocking shield One 30W halogen bulb One 100W mercury bulb One dust cover Color filters Two power cordsOne C-mount TEC USB camera One power adapter One AC power cord (North America plug) One USB 3.0 cable One software CD One heavy-duty case
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