Calmodulin-tag Binding/ Wash Buffer Catalog number: B2016319 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 50 mL Molecular Weight or Concentration: N/A Supplied as: Solution Applications: a molecular tool for various biochemical applications Storage: RT Keywords: Calmodulin Binding/ Wash Buffer Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Li CQ, Ye P, Cao Zf, Wang H, Lu L, Nicastro P, Wood E, Robert JJ, Ouwehand WH, Hill F, López JA, Wardell MR. Expression of the amino-terminal domain of platelet glycoprotein Ib alpha: exploitation of a calmodulin tag for determination of its functional activity Protein Expr Purif. 2001 Jul;22(2):200-10. Chad JE, Eckert R. An enzymatic mechanism for calcium current inactivation in dialysed Helix neurones J Physiol. 1986 Sep;378:31-51. Terpe K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems Appl Microbiol Biotechnol. 2003 Jan;60(5):523-33. Murray G, Marshall MJ, Trumble W, Magnuson BA. Calmodulin-binding protein detection using a non-radiolabeled calmodulin fusion protein Biotechniques. 2001 May;30(5):1036-42. Lin L, Liu C, Nayak BC, He W, You M, Yuchi Z. A two-step purification strategy using calmodulin as an affinity tag J Chromatogr A. 2018 Apr 6;1544:16-22. Mukherjee S, Ura M, Hoey RJ, Kossiakoff AA. A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin-Calmodulin Binding Peptide Interaction J Mol Biol. 2015 Aug 14;427(16):2707-25. Zhao X, Li G, Liang S. Several affinity tags commonly used in chromatographic purification J Anal Methods Chem. 2013;2013:581093. Klein W. Calmodulin-binding peptide as a removable affinity tag for protein purification Methods Mol Biol. 2003;205:79-97. Li Y. Commonly used tag combinations for tandem affinity purification Biotechnol Appl Biochem. 2010 Feb 15;55(2):73-83.
