T25 Cell Culture Flasks with Vented Cap NonTreated Sterile

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Description Biofargo cell culture flasks feature flat surfaces that offer large culture area and promotes growth. Vented caps with 0.2μm filter promote ventilation and prevent cont amination. Angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: T25, T75, and T175. Specifications Colour Flask: clear Cap: White Material Flask: PS Cap: HDPE Sterilization Irradiation sterilization Volume Growth area: 25cm2 Max. volume: 50mL Treatment Non-Treated Quality Control Raw Material-Control: physical testing Product-Control: testing of attributive and variable characteristics in accordance with the valid specification Features Vented caps with 0.2μm filter, promoting ventilation and preventing contamination. Angled bottle neck designed for pipettes and cell scrapers. Measurements and annotation area on both sides. Sterilized self-sealing bag to prevent contamination. Easily stackable. Gamma sterilized, DNase-free, RNase-free. Applications Not TC-Treated (Suspension Treatment): Use Scenario: Suitable for suspension cell culture, offering an adjustable culture environment and convenient operation. Features: Non-TC-treated containers are ideal for maintaining the growth and reproduction of suspended cells. Frequently Asked Questions What is a T25 cell culture flask used for? A T25 cell culture flask is designed for the growth, maintenance, and expansion of suspension mammalian cells. With a 25 cm² growth surface, it is commonly used for routine suspension cell culture, cell line maintenance, protein expression, and preparation of cells for downstream assays. What does Non-Treated mean on a cell culture flask? Non-Treated refers to a cell culture flask without tissue culture surface treatment. The untreated hydrophobic surface is suitable for suspension cell cultures or applications where cell attachment is not required. What is the growth area of a T25 flask? A T25 flask provides a growth surface area of 25 cm² and a maximum volume of 50 mL. This size is commonly used for routine suspension cell culture and offers convenient handling in the laboratory. What is the recommended working volume for a T25 cell culture flask? Although the maximum volume is 50 mL, most cell culture protocols recommend using approximately 5–10 mL of culture medium in a T25 flask. The optimal volume depends on the cell type and specific experimental requirements. What is the difference between a vented cap and a sealed cap cell culture flask? A vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required. Why is a 0.2 μm vent filter important in cell culture flasks? The 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators. Can this T25 flask be used for suspension cell culture? Yes. The non-treated surface is suitable for suspension cell lines that do not require attachment for growth. It is commonly used for suspension mammalian cells and other applications where cell adhesion is unnecessary. Are Biofargo T25 cell culture flasks sterile? Yes. Biofargo T25 Cell Culture Flasks are individually packaged and sterilized by irradiation. The flasks are supplied ready to use and are suitable for aseptic cell culture applications. Are these cell culture flasks DNase-free and RNase-free? Yes. Biofargo T25 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows. What material is used to manufacture the flask? The flask body is made from high-clarity polystyrene (PS), providing excellent visibility for microscopic observation and routine monitoring of cell cultures. The cap is made of HDPE. Can these T25 flasks be stacked during incubation? Yes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations. What types of applications are T25 non-treated cell culture flasks commonly used for? T25 non-treated cell culture flasks are widely used for suspension cell culture, hybridoma culture, protein expression, bioprocess development, cell maintenance, and general life science research where cell attachment is not required. T25,T25 flask,t-25 flask Show more