Cell Lysis Buffer Catalog number: B2014599 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 50 mL Molecular Weight or Concentration: N/A Supplied as: Solution Applications: molecular tool for various biochemical applications Storage: 2-8°C Keywords: Cell Lysis Buffer Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Peach M, Marsh N, Miskiewicz EI, MacPhee DJ. Solubilization of proteins: the importance of lysis buffer choice Methods Mol Biol. 2015;1312:49-60. Peach M, Marsh N, Macphee DJ. Protein solubilization: attend to the choice of lysis buffer Methods Mol Biol. 2012;869:37-47. Lu YA, Liao CT, Raybould R, Talabani B, Grigorieva I, Szomolay B, Bowen T, Andrews R, Taylor PR, Fraser D. Single-Nucleus RNA Sequencing Identifies New Classes of Proximal Tubular Epithelial Cells in Kidney Fibrosis J Am Soc Nephrol. 2021 Oct;32(10):2501-2516. Hughes CS, Moggridge S, Müller T, Sorensen PH, Morin GB, Krijgsveld J. Single-pot, solid-phase-enhanced sample preparation for proteomics experiments Nat Protoc. 2019 Jan;14(1):68-85. Tang Z, Takahashi Y. Analysis of Protein-Protein Interaction by Co-IP in Human Cells Methods Mol Biol. 2018;1794:289-296. Pearson BL, Posselt LP, Henzel KS, Ehninger D. Limited efficacy of somatic cell lysis buffer to purify laboratory mouse sperm Epigenomics. 2018 Jun;10(6):689-694. Nielsen KH. Protein expression-yeast Methods Enzymol. 2014;536:133-47. Oh H, Siano B, Diamond S. Neutrophil isolation protocol J Vis Exp. 2008 Jul 23;(17):745. Najar M, Rodrigues RM, Buyl K, Branson S, Vanhaecke T, Lagneaux L, Rogiers V, De Kock J. Proliferative and phenotypical characteristics of human adipose tissue-derived stem cells: comparison of Ficoll gradient centrifugation and red blood cell lysis buffer treatment purification methods Cytotherapy. 2014 Sep;16(9):1220-8.
