CENTRI-SPIN 10 Catalogue Numbers: CS-100 (Box of 20 Columns), CS-101 (Box of 50 Columns) Sizes: Box of 20 Columns (CS-100), Box of 50 Columns (CS-101) Protocol Available Here. Centri-Spin 10 spin columns are used for: electroporation nick translation binding applications FISH transfection probe labeling gene products signal transduction differential display antibody labeling transformation studies protein labeling receptor analysis solid phase array desalting. Centri-Spin 10 spin columns are used for the fast and efficient purification of larger molecules (proteins, nucleic acids, complex carbohydrates, etc.), from small molecules (nucleotides, buffer salts, etc.) The Centri-Spin 10 gel will provide excellent recovery of DNA fragments with sizes greater than 10 base pairs or 10-mer, while removing > 98 % of salts, NTP's and other unwanted low-molecular-weight impurities. Proteins, peptides, and protein conjugates > 5 kD can also be efficiently separated/desalted from unwanted low-molecular-weight impurities. The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Spin 10 unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose. The column gel is hydrated with either reagent-grade water or a suitable buffer, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the interstitial fluid. After your sample is applied, the column is spun again to remove the low-molecular-weight components, leaving you not only with a purified sample, but one exchanged into the buffer of your choice. CENTRI-SPIN 10 COLUMNS ALSO CAN BE USED FOR THE FOLLOWING APPLICATIONS: Purification of primers or probes > 10 bases Purification of biotinylation reactions Purification of protein conjugates in: iodination cross-linking fluorescence labeling haptenation Desalting / purification / buffer exchange of peptides or proteins > 5 kD prior to mass spectroscopy. These columns are far superior – in their ease of use, speed, and non-toxicity – to such common techniques as phenol/chloroform extraction, ethanol precipitation, dialysis and ultrafiltration. ADVANTAGES OF CENTRI-SPIN 10: Processed faster than ultrafiltration columns Rapid and efficient separations Buffer is not preselected Convenient 20–50 µL sample size Dry gel format Room Temperature Storage Microcentrifuge format BENEFITS OF CENTRI-SPIN 10: Reliable and consistent Time-saving and convenient Enhanced stability Extended shelf-life No special equipment required Extremely versatile
