Description The Human Urine cfDNA Extraction Kit (Cat. CF-DNA-301) is designed for high-efficiency isolation of circulating cell-free DNA (cfDNA) from up to 50 mL of urine in a single preparation. The kit utilizes an innovative double-layer column technology that maximizes cfDNA recovery while eliminating genomic DNA contamination from lysed white blood cells. The eluted cfDNA is highly pure and immediately ready for downstream molecular applications, including next-generation sequencing (NGS), qPCR, digital PCR, and methylation analysis. This kit is particularly suited for applications requiring large input volumes, such as early-stage cancer detection or liquid biopsy studies where cfDNA abundance is low. Performance Specifications Parameter Specification Sample volume Up to 50 mL urine cfDNA fragment size 80–500 bp Recovery rate ≥90% Yield >20 μg total cfDNA Purity Direct-use for NGS / PCR Genomic DNA contamination None detectable (WBC gDNA retained on membrane) Format 50 Preps Applications This high-capacity urine cfDNA extraction kit is optimized for: Early cancer detection & minimal residual disease (MRD)(large input volume improves sensitivity for low-abundance markers) Liquid biopsy & non-invasive genomic profiling Urothelial carcinoma screening Prenatal screening (urine-based cfDNA research) Transplant rejection monitoring Infectious disease monitoring via urinary cfDNA Epigenetic and methylation assays Biomarker discovery from low-input urine samples Large-volume capability significantly enhances assay sensitivity, making the CF-DNA-301 especially suitable for early-stage or low-abundance cfDNA studies where typical 1–5 mL plasma inputs are insufficient. Sample Volumes Kit Variant Sample Volume #301 Up to 50ml of Urine #302 Up to 5ml of Plasma #303 Up to 0.7ml of Plasma Storage Store at RT (20-25C). Related Products (Click Here) #302 cf-DNA Extraction Kit (Click Here) #303 cf-DNA Extraction Kit Product Manual User Manual This product is intended for research use only.
