Description Biofargo Chloroform: isoamyl alcohol (24:1) is a premixed, biotech-grade solvent mixture designed for efficient nucleic acid purification, enabling seamless separation of proteins from DNA/RNA samples. This high-purity reagent stabilizes the unstable phase interface during extractions, ensuring maximal recovery of intact nucleic acids for critical molecular biology applications. Specifications Product Name Chloroform: isoamyl alcohol (24:1) SKU CB0351 Grade Biotech Grade Composition Chloroform / Isoamyl alcohol mixture Appearance Clear, colorless liquid Specific Gravity 1.1446 - 1.463 Water Content Low water concentration specification Storage Temperature Room Temperature (RT), protect from moisture Shipping Condition Ships from our warehouse under regulated safety transport regulations. Features Ready-to-Use Precision: Premixed at a strict 24:1 ratio to prevent alignment variation and save preparation time. Biotech Grade Quality: High-purity formula with low water content to protect sensitive nucleic acid samples from degradation. Optimized Phase Separation: Isoamyl alcohol acts as an anti-foaming agent, ensuring a clear and stable organic/aqueous interface. Guaranteed Traceability: Every batch is fully verified with an available Certificate of Analysis (COA) and Safety Data Sheet (SDS). Applications This product is optimized for a wide array of genomic and transcriptomic research protocols, including: Phenol-chloroform DNA extraction and recovery from polyacrylamide gels. RNA isolation and purification compatible workflows. Removal of trace mineral oil from post-PCR amplification reaction mixtures. Oligonucleotide purification and plasmid DNA isolation protocols. How to Use Note: The following instructions are for general reference only. Please optimize the protocol based on your specific experimental design. Phase Separation: Add an equal volume of Chloroform:isoamyl alcohol (24:1) to your aqueous nucleic acid sample previously treated with phenol. Homogenization: Vortex the mixture vigorously for several seconds to form a complete emulsion. Centrifugation: Spin the mixture under recommended laboratory speeds and temperature to cleanly separate the phases. Recovery: Carefully aspirate the upper aqueous phase containing the purified DNA/RNA into a fresh, sterile tube, leaving the interphase proteins behind. Safety Signal Word: Danger Hazard Statements: Harmful if swallowed | Causes skin irritation | Causes serious eye damage | Toxic if inhaled | May cause drowsiness or dizziness | Suspected of causing cancer | Suspected of damaging fertility or the unborn child. Precautionary Statements: Do not breathe dust/fume/gas/mist/vapors/spray. Wear protective gloves/clothing/eye protection/face protection. IF INHALED: Remove person to fresh air. IF IN EYES: Rinse cautiously with water for several minutes. Target Organs: Central nervous system, Liver, Kidney. Documents COA SDS FAQ Q1: Why is isoamyl alcohol added to chloroform at a 24:1 ratio? A: Chloroform is efficient at denaturing proteins, but it tends to cause significant foaming during shaking or vortexing. Isoamyl alcohol acts as an anti-foaming agent, ensuring optimal phase separation and stabilization of the interface between the aqueous and organic layers. Q2: How should Biofargo Chloroform:isoamyl alcohol (24:1) be stored? A: It should be stored at room temperature (RT) in a dry, well-ventilated area. Keep the container tightly sealed and protected from moisture and direct ignition sources to retain peak quality. Q3: Is this product suitable for both DNA and RNA isolation? A: Yes. This biotech grade mixture is strictly prepared and quality controlled, making it fully compatible with both DNA extraction and sensitive RNA extraction workflows where RNase inhibition is necessary. Q4: Does this product require specialized hazardous shipping? A: Yes. Due to its classification as an acute toxic chemical solution, it is shipped as a regulated hazardous material from our warehouse following strict safety compliance guidelines. Q5: Can I request a lot-specific Certificate of Analysis (COA)? A: Absolutely. You can download the general technical specification sheet directly from this page, or contact our customer support team with your specific lot number to obtain the complete actual test results.
