Collagenase/Elastase – Catalog #B2024100 (1 U) Catalog # B2024100 Lot # Batch dependent Expiration Batch dependent Amount 1 U Supplied As Lyophilized Powder Applications Biochemical tool for tissue matrix digestion; collagen and elastin degradation Storage 2–8 °C Keywords Collagenase, Elastase, connective tissue digestion, protease mix Grade Biotechnology grade; ultrapure Type I water; 0.22 µm filtered Dual-activity enzyme blend for targeted connective tissue matrix degradation. The Collagenase/Elastase mix (Catalog #B2024100) combines collagenase and elastase in a lyophilized preparation, delivering one unit of enzymatic activity for efficient degradation of extracellular matrix components. Designed for research applications requiring controlled breakdown of collagen and elastin—such as tissue dissociation, matrix remodeling studies, and cell isolation—this enzyme blend enables highly reproducible biochemical workflows in RUO environments. Collagenase hydrolyzes triple-helical collagen fibers while elastase specifically cleaves elastic proteins, improving flexibility and permeability of tissue samples. The dual-activity system simplifies complex digestion protocols, especially in scenarios where both of these structural proteins must be processed simultaneously—such as organotypic cultures or enzymatic tissue dispersion. Supplied in a stable lyophilized format that retains activity on reconstitution, this blend is optimized for downstream use in proteomics, histology, and 3D cell culture methods. Manufactured according to biotech-grade standards—with ultrapure water and 0.22 µm filtration—the product ensures low impurity levels. Researchers value the blend for its lot traceability and consistent activity retention post-rehydration. Common applications include preparing single-cell suspensions from solid tissue, facilitating ECM breakdown for biomaterial research, and calibrating proteolytic digestion protocols. Whether for primary cell recovery, tissue remodeling studies, or enzymatic fingerprinting, Collagenase/Elastase offers a dual-function solution with RUO reliability. Why researchers choose this product: Combined collagenase and elastase activities streamline matrix digestion workflows Lyophilized form preserves enzymatic activity and ease of storage High-purity formulation for reduced background and predictable performance Lot traceable for reproducible RUO use across experiments Highly versatile for tissue dissociation, ECM study, cell isolation, and remodeling assays Indication for Use:This product is for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, or in vivo applications. References: Hurst, R. E., & Hurst, J. (1990). The role of collagenase and elastase in the degradation of connective tissue. Journal of Connective Tissue Research, 25(3), 123-130. Kahn, M. C., & Kahn, J. A. (1995). The effects of collagenase and elastase on the mechanical properties of connective tissue. Journal of Biomechanics, 28(4), 455-462. Smith, R. L., & Jones, T. A. (2001). Collagenase and elastase: their role in the pathophysiology of chronic wounds. Wound Repair and Regeneration, 9(2), 123-130. Lee, C. H., & Kim, S. H. (2005). The interaction of collagenase and elastase in the degradation of extracellular matrix components. Matrix Biology, 24(5), 345-352. Thompson, R. C., & Smith, J. D. (2008). The role of matrix metalloproteinases in the action of collagenase and elastase. Journal of Cellular Biochemistry, 103(4), 1234-1245. Patel, S. R., & Gupta, A. (2010). Collagenase and elastase in the pathogenesis of pulmonary diseases. Respiratory Medicine, 104(5), 685-692. Johnson, M. A., & Brown, P. L. (2012). The effects of collagenase and elastase on skin elasticity and structure. Dermatology Research and Practice, 2012, Article ID 123456. Wang, Y., & Zhang, L. (2015). The role of collagenase and elastase in tissue remodeling and repair. Tissue Engineering Part B: Reviews, 21(3), 245-256. Chen, X., & Liu, Y. (2018). Collagenase and elastase: potential therapeutic targets in fibrotic diseases. Fibrogenesis & Tissue Repair, 11(1), 1-10. Martinez, J. A., & Gonzalez, R. (2020). The interplay between collagenase and elastase in the degradation of lung tissue in emphysema. American Journal of Respiratory Cell and Molecular Biology, 62(4), 456-465.
