Overview TriAltus’ LbCas12a protein is a highly pure, tag-free CRISPR Type V-A nuclease optimized for genome editing and CRISPR diagnostic workflows. Derived from Lachnospiraceae bacterium and expressed in E. coli, LbCas12a recognizes TTTV PAM sequences and generates staggered 5’ overhangs, enabling precise DNA target engagement and multiplex editing capabilities. Also known as: LbCas12a nuclease, CRISPR Cas12a protein, Cpf1 nuclease, Lachnospiraceae Cas12a, Cas12a CRISPR nuclease Why Purity and Activity Matter for Cas12a Cas12a (Cpf1) differs from Cas9 in both mechanism and workflow, placing added importance on enzyme purity and consistent activity for reproducible results. Cas12a relies on a single crRNA (without a tracrRNA), recognizes TTTV PAM sequences, and introduces staggered DNA cuts, making RNP assembly and cleavage efficiency particularly sensitive to inactive protein populations or impurities. In multiplex genome editing and CRISPR diagnostic workflows—where Cas12a’s collateral cleavage behavior is often leveraged—protein quality can directly influence signal clarity, background activity, and experiment-to-experiment consistency. High-purity, well-characterized Cas12a supports reliable crRNA binding and predictable cleavage performance, enabling more consistent results across diverse CRISPR applications. Bulk Supply & Program Support TriAltus supports bulk supply and tailored formats for organizations requiring dependable, long-term access to critical enzymes. Production under ISO 13485–compliant processes and the CLīM™ purification platform enable batch consistency and efficient cost-of-goods at scale, supporting volume-based pricing for sustained programs. Contact TriAltus to discuss volume, documentation, packaging, and supply planning. Key Benefits Tag-free LbCas12a for reduced non-specific interactions Class 2, Type V-A CRISPR nuclease with precise PAM targeting Dual nuclear localization signals (NLS) enhance nuclear delivery Ideal for multiplex editing, sensitive detection, and diagnostics workflows Specifications PURITY | >90% ENZYME SOURCE | Lachnospiraceae bacterium Cas12a is expressed in E. coli with two C-terminal nuclear localization signals (NLS) and is purified with our CLīM technology. STORAGE/SHIPPING CONCENTRATION | ~ 5 mg/mL STORAGE BUFFER | 10 mM Tris-Cl pH 8.0, 250 mM NaCl, 50% glycerol RECOMMENDED STORAGE CONDITIONS | -80°C EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. Produced under ISO 13485–compliant processes. LbCas12a Data Sheet SKU List 100 µg: 40-1120 | 500 µg: 40-1125 | 1 mg: 40-1130 | Bulk: Inquire Applications LbCas12a supports diverse CRISPR workflows: CRISPR–Cas12a RNP Genome Editing LbCas12a forms stable RNP complexes with crRNA alone, permitting precise genome editing at TTTV PAM sites and enabling simultaneous editing of multiple targets in a single experiment. CRISPR Diagnostics & Detection Beyond gene editing, Cas12a’s collateral ssDNA cleavage activity underlies CRISPR-based detection strategies, including sensitive nucleic acid assays and point-of-care diagnostics. In Vitro Cleavage & Screening This protein is suitable for in vitro DNA cleavage and screening workflows, offering consistent performance for method development and comparative analyses. Learn More Learn how TriAltus achieves high purity using one-step CLīM™ purification, a high-affinity system designed to streamline recombinant protein production while maintaining consistent quality. Explore peer-reviewed publications describing the underlying purification platform and protein production methods used across TriAltus enzyme products. For related CRISPR enzymes, view the AapCas12b Protein page to compare alternative Cas12 family nucleases and workflow options. To compare nuclease selection across CRISPR workflows, see the Purified CRISPR-Cas9 Protein page FAQs Is LbCas12a tag-free? Yes, the protein is supplied without affinity tags for streamlined RNP formation. What guides does LbCas12a use? LbCas12a complexes with a single crRNA sequence, no tracrRNA required. What PAM does LbCas12a recognize? TTTV (V = A/G/C) PAM sequences for DNA targeting.
