DAB Substrate Kit (Catalog number: 003836) (Distributor and quantity discounts available, please send an email [email protected] to order. For common questions and answers, please check our FAQ.) Bioenno is currently selling the DAB Substrate Kit (Catalog Number: 003836). DAB (3,3’-diaminobenzidine) is a chromogen popularly used in immunohistochemistry, immunocytochemistry, and immunoblotting. The Bioenno DAB Substrate Kit is ideal for immuno-labeling on tissue sections and cells to detect the activity of peroxidase (HRP). The brown DAB-HRP reaction product is alcohol and heat resistant, and is also suitable for electron microscopy (EM). The kit contains all of the necessary reagents to prepare about 330 ml of substrate working solution, and the stock solutions are contained in convenient dropper bottles. The kit can be stored in a dark area at 2-8ºC and is stable for 12 months. Production Description and Protocol DAB Substrate Kit Protocol Bioenno Tech has successfully used the DAB Substrate Kit, (employed together with the Bioenno BDHC Substrate Kit), for dual-labeling immunohistochemistry. Figure: Bioenno DAB Substrate Kit employed together with the Bioenno BDHC Substrate Kit (A,B) The Bioenno DAB Substrate Kit was used in standard avidin-biotin-peroxidase immunochemistry, which was performed on free-floating sections. (C) Dual-labeling immunohistochemistry using the DAB Substrate Kit (brown color) and BDHC Substrate Kit (blue color). DAB was used as the first chromogen and BDHC was the second one. The boxed areas were magnified (63x) to highlight immuno-labeled neurons. Images were taken from adult rat brains. Warranty: 12 months from the date of purchase. Return Policy: Bioenno Tech’s return policy for this product is 90 days from the date of purchase. Free Technical Support: Email your questions to [email protected] Immunohistochemistry Substrate Kits cited 2017 Running-induced memory enhancement correlates with the preservation of thin spines in the hippocampal area CA1 of old C57BL/6 mice Benke Xu, Anbang Sun, Yun He, Feng Qian, Lian Liu, Yuncai Chen, Huanmin Luo(2017), Neurobiology of Aging,52, 106-116Link: http://www.sciencedirect.com/science/article/pii/S0197458017300027 2016 Dual and opposing roles of microRNA-124 in epilepsy are mediated through inflammatory and NRSF-dependent gene networksBrennan GP, Dey D, Chen Y, Patterson KP, Magnetta EJ, Hall AM, Dube CM, Mei YT, Baram TZ (2016). Cell Reports, 14(10), 2402-2412.Link: http://www.ncbi.nlm.nih.gov/pubmed/25134728 brain tissue, dendrite, dendritic spine, GFP, gfp, golgi cox, golgi impregnation, golgi stain, golgi’s method, impregnation, rapid golgi, spine, staining, super golgi, supergolgi, synapse
