Denhardt’s Solution (50x) Catalog number: B2014574 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 50 mL Molecular Weight or Concentration: N/A Supplied as: Solution Applications: molecular tool for various biochemical applications Storage: −20°C Keywords: Denhardt’s Solution (50x) Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Lee GM, Rasch EM, Musich PR. Improved in situ hybridization and G-banding by pretreatment with Denhardt’s solution and gelatin-chrome alum Histochem J. 1985 Nov;17(11):1221-33. Cannon G, Heinhorst S, Weissbach A. Quantitative molecular hybridization on nylon membranes Anal Biochem. 1985 Aug 15;149(1):229-37. Hunger HD, Speer A, Flachmeier C, Hanke R, Behrendt G, Coutelle C. Use of cyanuric chloride-activated paper for detection of subpicogram quantities of specific DNA sequences and its application to linked restriction fragment length polymorphism analysis in a Duchenne muscular dystrophy affected family Anal Biochem. 1987 Aug 15;165(1):45-55. Common buffers, media, and stock solutions Curr Protoc Hum Genet. 2001 May;Appendix 2:Appendix 2D. Lee GM, Rasch EM, Musich PR. Improved in situ hybridization and G-banding by pretreatment with Denhardt’s solution and gelatin-chrome alum Histochem J. 1985 Nov;17(11):1221-33. Cassey J, Armstrong P, Colyvas K, Stanger R. Comment on ‘Prevention of intraoperative hypothermia…’ Witt L, Denhardt N, Eich C et al Paediatr Anaesth. 2013 Oct;23(10):970. Ford HL, Pardee AB. Cancer and the cell cycle J Cell Biochem. 1999;Suppl 32-33:166-72. O’Regan A, Berman JS. Osteopontin: a key cytokine in cell-mediated and granulomatous inflammation Int J Exp Pathol. 2000 Dec;81(6):373-90. Denhardt DT. The single-stranded DNA phages CRC Crit Rev Microbiol. 1975 Dec;4(2):161-223.
