/* ========================= Biofargo Table SOP (Embedded) ========================== */ .biofargo-wrap .biofargo-table-wrap{ overflow-x:auto; -webkit-overflow-scrolling:touch; border:1px solid #eee; border-radius:10px; padding:6px; background:#fff; margin-top:15px; } .biofargo-wrap table.biofargo-table{ width:100%; border-collapse:collapse; font-size:14px; min-width:640px; background:#fff; } .biofargo-wrap table.biofargo-table th, .biofargo-wrap table.biofargo-table td{ border:1px solid #e9ecef; padding:15px; vertical-align:middle; white-space:normal; word-break:break-word; } .biofargo-wrap table.biofargo-table thead th{ background:#f2f2f2; color:#000; font-weight:700; text-align:left; } .biofargo-wrap table.biofargo-table tbody td:first-child{ background:#f8f9fa; font-weight:600; width:30%; } Description In routine mammalian cell culture workflows, researchers often face inconsistent cell growth, unstable pH conditions, and reduced reproducibility when using low-quality or poorly formulated basal media. These issues can directly affect downstream applications such as cell line expansion, primary cell culture, and protein expression studies. This DMEM High Glucose cell culture medium is a sterile filtered, high-performance basal medium designed to support reliable and reproducible in vitro cell growth. It provides a balanced nutrient environment optimized for high metabolic demand cells, helping reduce variability commonly observed in standard cell culture systems. Formulated according to widely adopted mammalian cell culture medium standards (including Gibco and Sigma-Aldrich DMEM formulations), it supports stable growth of adherent and primary cells when supplemented with fetal bovine serum (FBS). The high glucose concentration ensures sufficient energy supply for rapidly proliferating cells, while buffering components maintain physiological pH during CO₂ incubation. This medium is suitable for researchers who require consistent, endotoxin-tested, and reproducible performance in both routine cell maintenance and advanced experimental workflows. Specification Full name DMEM Medium, High Glucose Applications Mammalian cell culture using sterile filtered cell culture medium Primary cell culture including hepatocytes and endothelial cells Adherent cell line expansion for research-grade DMEM High Glucose workflows Serum-supplemented culture systems (FBS-dependent growth conditions) Fibroblast and epithelial cell culture in standard DMEM formulation High-glucose metabolic cell culture for fast-growing mammalian cells Cell culture applications requiring endotoxin tested media consistency Tissue culture systems comparable to Gibco DMEM and Sigma-Aldrich DMEM standards Cell line maintenance and routine passaging in basal medium for cell culture In vitro cell proliferation and viability studies Transfection-preparation culture conditions for mammalian expression systems Biotechnology and academic research requiring reproducible cell growth medium Components D-Glucose (4.5 g/L) Sodium bicarbonate (3.7 g/L) Sodium pyruvate (0.11 g/L) HEPES (6.0 g/L) L-Glutamine (4 mM) Phenol Red Storage 2~8°C Frequently Asked Questions What is DMEM High Glucose used for in cell culture? DMEM High Glucose is a widely used mammalian cell culture medium designed for adherent cell lines and primary cells. It provides a high-energy environment that supports rapid cell growth and is commonly used in cell line expansion, primary cell culture, and endothelial or hepatocyte research workflows. Why is high glucose DMEM preferred for certain cell types? The high glucose formulation (4.5 g/L glucose) supports energy-demanding cells with elevated metabolic activity. This makes it suitable for mammalian cell culture systems requiring consistent growth performance, especially in serum-supplemented (FBS) conditions. Does this DMEM contain L-glutamine and sodium pyruvate? Yes. This DMEM formulation includes L-glutamine and sodium pyruvate, which are essential for amino acid metabolism and energy production. These components are standard in many sterile filtered cell culture media used for research-grade mammalian cell culture. Is this medium sterile and endotoxin tested? Yes. The product is sterile filtered and USP sterility tested, with endotoxin levels ≤5 EU/mL. This ensures reliability in sensitive mammalian cell culture applications and maintains consistency comparable to leading cell culture media manufacturers such as Gibco and Sigma-Aldrich. What serum should be used with DMEM High Glucose? This medium is typically supplemented with 10% fetal bovine serum (FBS) to support optimal cell growth, attachment, and proliferation in mammalian cell culture systems. Can this medium be used for primary cell culture? Yes. DMEM High Glucose is commonly used for primary cell culture, including hepatocytes, fibroblasts, and endothelial cells, providing a stable and nutrient-rich environment for sensitive cell types.
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