/* ========================= Biofargo Product Page SOP ========================== */ .biofargo-wrap{ max-width:1000px; margin:0 auto; padding:10px; font-family:'Segoe UI', Roboto, Helvetica, Arial, sans-serif; color:#333; line-height:1.7; } .biofargo-wrap .biofargo-sec{ margin-bottom:32px; } .biofargo-wrap .biofargo-h2{ padding-left:15px; color:#0056b3; font-size:20px; text-transform:uppercase; margin:0 0 15px 0; } .biofargo-wrap .biofargo-card{ background:#f9f9f9; padding:18px; border-radius:10px; border:1px solid #eee; } .biofargo-wrap ul{ padding-left:22px; } .biofargo-wrap li{ margin:8px 0; } /* ========================= Table Style (Biofargo SOP) ========================== */ .biofargo-table-wrap{ overflow-x:auto; -webkit-overflow-scrolling:touch; } .biofargo-wrap table.biofargo-table{ width:100%; border-collapse:collapse; font-size:14px; min-width:640px; } .biofargo-wrap table.biofargo-table th, .biofargo-wrap table.biofargo-table td{ border:1px solid #ddd; padding:12px; vertical-align:middle; word-break:break-word; } .biofargo-wrap table.biofargo-table thead th{ background:#f2f2f2; font-weight:700; } .biofargo-wrap table.biofargo-table tbody td:first-child{ font-weight:700; background:#f8f9fa; } Description The 2K Plus DNA Marker is composed of 8 linear double stranded DNA bands, namely: 100, 250, 500, 750, 1000, 1200, 1500, and 2000 bp, with 1 bp bands displayed as bright bands. This product was previously listed under catalog number P310147-0500 and is now updated to P310147-500ul for improved product management. Specification Parameter Specification Package 500 μL Full name DNA Marker (100–2000 bp) Composition 1 mM Tris-HCl (pH 8.0), 1 mM EDTA, 0.2% Bromophenol Blue, 0.1% Xylene Cyanol FF, and 5% Glycerol. Storage Buffer 1 mM Tris-HCl (pH 8.0), 1 mM EDTA, 0.2% Bromophenol Blue, 0.1% Xylene Cyanol FF, and 5% Glycerol. Color Blue Features Pre-mixed DNA ladder with loading dye for direct use in agarose gel electrophoresis. Sharp and uniform band intensity for accurate DNA fragment size estimation. Stable linear double-stranded DNA fragments covering 100–2000 bp range. Compatible with routine molecular biology workflows including PCR product verification. Applications Widely used as a DNA ladder / DNA marker for agarose gel electrophoresis in molecular biology laboratories. Ideal for PCR product size estimation and DNA fragment size analysis in routine DNA electrophoresis workflows. Supports cloning verification, restriction enzyme digestion analysis, and recombinant DNA screening. Suitable for molecular weight DNA marker comparison in gel electrophoresis DNA ladder systems. Commonly applied in nucleic acid separation, gene fragment identification, and standard DNA size determination experiments. Recommended for DNA fragment sizing in 1%–2% agarose gels for both routine and high-resolution DNA analysis. Storage -20°C FAQ What is a DNA ladder used for in gel electrophoresis? A DNA ladder (or DNA marker) is used as a molecular weight reference to estimate DNA fragment size during agarose gel electrophoresis. It enables accurate comparison of PCR products and digested DNA samples. How do I choose the correct DNA marker for PCR products? For PCR product analysis, a 100–2000 bp DNA ladder is ideal because it covers the typical range of amplified DNA fragments used in cloning and gene analysis workflows. Why are DNA ladder bands sometimes faint or uneven? Weak or uneven bands may result from improper storage, repeated freeze-thaw cycles, or incorrect gel concentration in agarose gel electrophoresis systems. What is the difference between a DNA ladder and a DNA marker? Both terms are commonly used interchangeably. They refer to a mixture of DNA fragments of known sizes used as molecular weight standards in DNA electrophoresis experiments. Can this DNA ladder be used for cloning verification? Yes, it is suitable for cloning verification, restriction digestion analysis, and DNA fragment size determination in molecular biology applications.
