Description Our dNTPs are analytically tested to ensure a purity of >99% by HPLC, guaranteeing optimal performance in demanding PCR amplification and DNA synthesis workflows. This ultrapure nucleotide mix provides balanced concentrations of dATP, dCTP, dGTP, and dTTP, ensuring high efficiency for PCR reagents, RT-PCR, and molecular biology applications requiring consistent polymerase performance. Specification Parameter Specification Purity ≥ 99% (HPLC) Total Product Size 0.5 mL Individual Container Size 0.5 mL Number of Containers 1 Refrigeration Requirements Freezer Shipping Conditions ICE UNSPSC Code 12352208 UNSPSC Category Nucleic Acid Bases Grade Biotech DNase/RNase Not detected (nuclease-free) Storage -15 to -20°C Sterile No Stock Usually in stock Summary DNase and RNase activity: not detected. Designed for PCR amplification, DNA synthesis, and molecular biology workflows requiring reliable nucleotide substrates. PCR Suitability This dNTP mix contains equimolar concentrations of four deoxynucleotide triphosphates: 10 mM dATP 10 mM dCTP 10 mM dGTP 10 mM dTTP Validated in PCR amplification systems at a final concentration of 200 μM, producing a clear ~500 bp DNA fragment, confirming compatibility with standard DNA polymerase-based PCR reagents and high-fidelity amplification workflows. Quality Control Endonuclease / Exonuclease Test No detectable degradation of λ DNA fragments after 16-hour incubation, confirming absence of contaminating nucleases that could interfere with DNA polymerase reactions. Nickase Activity Test No conversion of supercoiled plasmid DNA observed, ensuring structural stability in molecular biology grade PCR nucleotide applications. RNase Contamination Test No degradation of RNA substrates detected, confirming suitability for RT-PCR and nucleic acid amplification workflows. Frequently Asked Questions What is a dNTP mix used for in PCR amplification? A dNTP mix provides the four nucleotide building blocks (dATP, dCTP, dGTP, dTTP) required for DNA polymerase activity in PCR reagents. It is essential for DNA amplification, RT-PCR, and molecular biology workflows. Is this dNTP mix suitable for high-fidelity PCR and long-range PCR? Yes. The ultrapure, nuclease-free formulation with ≥99% HPLC purity supports high-fidelity PCR, long-range PCR, and DNA synthesis applications where enzyme accuracy and nucleotide quality are critical. What does “ultrapure” mean for dNTP reagents? Ultrapure dNTPs refer to nucleotide triphosphates with minimal impurities, typically verified by HPLC purity testing. This reduces inhibition in DNA polymerase reactions and improves reproducibility in PCR amplification. Can this product be used for RT-PCR and qPCR workflows? Yes. This dNTP mix is compatible with RT-PCR and qPCR nucleic acid amplification workflows, supporting reverse transcription and quantitative DNA amplification reactions. How should dNTP mix be stored to maintain stability? Store at -15°C to -20°C in a freezer. Avoid repeated freeze-thaw cycles to maintain nucleotide stability and consistent performance in PCR and DNA synthesis reactions. Is this product free from nuclease contamination? Yes. Quality control testing confirms no detectable DNase or RNase activity, ensuring reliable performance in sensitive molecular biology applications such as PCR amplification and sequencing preparation. Documents Download Info Download SDS Technical Bulletin Disclaimer For laboratory research use only.
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