Description EndoTrap® is an affinity matrix intended for removal of lipopolysaccharide (LPS) from biological samples in aqueous solutions such as proteins, antibodies, cell extracts and nucleic acids. This multipurpose product portfolio covers a broad range of conditions with no special equipment or buffer requirements. Affinity chromatography for highly efficient endotoxin removal Removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate. Helps avoid artefacts caused by endotoxin contamination in sensitive in-vitro and in-vivo assays. Can be employed in both column or batch mode, by gravity flow or on fully automated liquid chromatography systems. EndoTrap® red is available as prefilled endotoxin removal columns or bulk endotoxin removal resin. EndoTrap® red can be employed in both batch and column mode and is specially developed for buffers containing calcium chelators and for low-salt conditions. Features Endotoxin binding capacity: 2,000,000 EU/ml Sample recovery rate: >95% Toxicity: Non-toxic, no cytotoxic effects Reusability: Re-use at least 3 times Suitable for low-salt conditions?: Yes pH sample range: 6-9 Buffers: Suitable for PBS, HEPES, Borate, TRIS, MOPS, MES, PIPES, Citrate, Acetate, Glycine and Carbonate buffers Format: Pre-filled gravity flow column or bulk resin Choosing between EndoTrap Red and HD Buffer Compatibility Endotoxin removal with EndoTrap® red works effectively in the pH range of 6-9 and NaCl concentrations in the range of 50-250 mM. Buffers such as HEPES, PBS, TRIS, MOPS, MES, PIPES and also Citrate, Acetate, Glycine and Carbonate buffers are recommended. EndoTrap® red can be used with chelators of divalent cations (like EDTA). Phosphate buffers with NaCl concentrations below 100 mM are recommended for EndoTrap® red. Products Available in EndoTrap® red Family Product Name Cat. No. Description EndoTrap® red 1/1 LET0001 Kit: 1 x 1 mL column, 25 mL 5x Equilibration Buffer, 25 mL 5x Regeneration Buffer EndoTrap® red 5/1 LET0002 Kit: 5 x 1 mL column, 125 mL 5x Equilibration Buffer, 125 mL 5x Regeneration Buffer EndoTrap® red 1 mL Column LET0034 1 mL resin in column (buffers not included) EndoTrap® red 5 LET0033 5 mL settled resin EndoTrap® red 10 LET0003 10 mL settled resin EndoTrap® red 50 LET0004 50 mL settled resin EndoTrap® red Buffer Kit LET0032 125 mL 5x Equilibration Buffer, 125 mL 5x Regeneration Buffer Empty Columns Large LET0019 for 2-10 mL resin (pack of 5 columns) Empty Columns Small LET0020 for 0.2-2 mL resin (pack of 5 columns) Disposable Polypropylene Funnel LET0018 pack of 5 funnels EndoGrade® Endotoxin-Free Glass Test Tubes (112 ct.) LET0021 112 endotoxin-free borosilicate glass test tubes with aluminum screw cap, capacity 5 ml, height 50 mm Resources EndoTrap red Datasheet EndoTrap red Material Safety Data Sheet (MSDS) FAQ about EndoTrap EndoTrap User Applications Related Products EndoTrap® HD Ilex Life Sciences LLC is an official distributor of EndoTrap® and EndoGrade® products manufactured by LIONEX GmbH.
