Description Our Fibronectin Human, E. coli Recombinant Protein is a single, non-glycosylated polypeptide chain containing 574 amino acids and has a molecular mass of 62.6 kDa. The Fibronectin is purified by proprietary chromatographic techniques.Fibronectin is useful for the induction of cell attachment to a variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or serum-free medium to a concentration of 10-50 µg/ml. Summary Fibronectin is an ubiquitous extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix. It plays a major role in many important physiological processes, such as embryogenesis, wound healing, hemostasis, thrombosis, blood clotting, and cell migration/adhesion. Fibronectin consists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix and 2) as a soluble disulphide linked dimer found in the plasma. The plasma form is produced by hepatocytes, and the ECM form is synthesized by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.Fibronectin's importance during cancer progression has further been shown. It represents an active element in the process of T cell activation in the immune cascade triggered by organ transplantation. Plasma fibronectin level is elevated in severe coronary artery disease. Increased plasma fibronectin levels are related with venous thromboembolism (VTE) particularly in males, and extend the probable association between biomarkers and risk factors for arterial atherothrombosis and VTE.Fibronectin also takes part as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. Fibronectin organizes cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane- bound Fibronectin receptors on cell surfaces. CAS Registry No. 86088-83-7 Type Recombinant Protein Source E. coli Purity Greater than 95.0% as determined by: Analysis by RP-HPLC. Analysis by SDS-PAGE. Formulation Sterile filtered white lyophilized (freeze-dried) powder. Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 5% Trehalose and 0.02% Tween-20. Reconstitution It is recommended to reconstitute the lyophilized Fibronectin in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Biological Activity Bioactivity was measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The recommended concentration in this application for this effect is typically 1-5 μg/cm2. Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5-50 μg/ml. Optimal concentrations will need to be determined for individual user applications. Shipping At ambient temperature. Upon receipt, store the product at the temperature recommended below. Storage/Expiration Lyophilized Fibronectin, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, Fibronectin should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. Usage This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive. Other Names Cold insoluble globulin, FINC, LETS, MSF, FN Resources Product Datasheet (PDS) Related Products Attachment Factors Fibronectin Human, Native Purified Protein Fibronectin Rat, Native Purified Protein
