Product Specifications: Item # 1301-F: Fluorescene Rabbit Anti-gp120 HIV-1 IIIB pAb IgG Mass/vial: Approx. 50ug IgG Vol/vial: 100ul Diluent: 10mM Tris, pH 8.0 Stabilizer: None Preservative: None Storage: -75°C F/P Ratio: 2 - 4 Physical State: Frozen Liquid Stability: At least 12 months at -75°C Minimum Dilution: 1 : 50 Applications: Immunohistocytology, Immunostaining/sorting, Dot Blots, FACS. Description: Fluorescene conjugated Rabbit anti-gp120 (HIV-1 IIIB) pAb IgG. Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography. Specificity: This fluorescene conjugated pAb IgG binds to native and recombinant HIV-1 gp120 in Dot Blot assays and stains cell surfaces in direct immunofluorescence assays. Biological Activity: None Application and Instruction for use: Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of cells may be performed in 1 : 50 dilution range. Dot Blot assays with purified gp120 may be performed at a minimum dilution of 1 : 100.
