Product Specifications: Item#1091-G:rgp41 HIV-1 MN Conjugated to Colloidal Gold Concentration: 25ug protein Volume/vial: 1ml Gold Conjugate Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; 0.02% NaN3 Storage: 4°C Stability: At least 3 months at 4°C Minimum Dilution: 1 : 50 Application: HIV-1 Diagnostics, Antigen Capture, Dot Blot Assay-Anti-gp41 Antibody Binding, Immunohistochemistry. Description: rgp41 HIV-1 MN conjugated to Colloidal Gold (45nm mean particle size). Coupling Ratio: OD 520nm = 10; protein content approximately 25µg. Specificity: Gold conjugated rgp41 binds to murine monoclonal antibodies of defined epitope specificity and human polyclonal antibodies as determined by dot blot assays. Biological Activity: Not determined. Application and Instructions for use: Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. Dot blot assays may be performed in 1 : 50 dilution range depending on the concentration of solid phase antibody in the test system. Antigen capture assays are performed with solid phase antigen in concentrations of 1ug or more using HIV-1 converted serum for antigen cross linking. 2. Immunohistochemical studies may be performed in 1 : 50 dilution range. Related Products: Item#: 1091-P Peroxidase-Conjugated rgp41 HIV-1 MN Application: Human diagnostics. Item#: 1091-F Flourescene-Conjugated rgp41 HIV-1 MN Application: FACS analysis, Histocytology. Item#: 1091-M Magnetically-Coupled rgp41 HIV-1 MN Application: Human diagnostics, Human B and T-cell panning
