Product Specifications: Item#1002-G: tat HIV-1 IIIB Conjugated to Colloidal Gold Concentration: See vial Volume/vial: 1ml Gold Conjugate Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; 0.02% NaN3 Storage: 4°C Stability: At least 3 months at 4°C Minimum Dilution: 1 : 50 Application: Dot Blot Assay-CD4 Binding, Dot Blot Assay-Anti-gp120 Antibody Binding, Immunohistochemistry. Description: tat HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size). Coupling Ratio: OD 520nm = 10; protein content approximately 25µg. Specificity: Gold conjugated tat binds to murine monoclonal antibodies of defined epitope specificity and human/rabbit polyclonal antibodies as determined by dot blot assays. Biological Activity: Not determined. Application and Instructions for use: Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. Dot blot assays may be performed in 1 : 100 dilution range depending on the concentration of solid phase CD4/Anti-gp120 in the test system. 2. Immunohistochemical studies may be performed in 1 : 50 dilution range. Related Products: Item#: 1002-P Peroxidase-Conjugated tat HIV-1 IIIB Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies. Item#: 1002-F Flourescene-Conjugated tat HIV-1 IIIB Application: FACS analysis, Histocytology. Item#: 1002-M Magnetically-Coupled tat HIV-1 IIIB
