Gram-Negative Lysis Buffer Solution Catalog number: B2016359 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 50 mL Molecular Weight or Concentration: N/A Supplied as: Solution Applications: a molecular tool for various biochemical applications Storage: RT Keywords: Gram-Negative Lysis Buffer Solution Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Ares M. Bacterial RNA isolation Cold Spring Harb Protoc. 2012 Sep 1;2012(9):1024-7. Glatter T, Ahrné E, Schmidt A. Comparison of Different Sample Preparation Protocols Reveals Lysis Buffer-Specific Extraction Biases in Gram-Negative Bacteria and Human Cells J Proteome Res. 2015 Nov 6;14(11):4472-85. Saha B, Saha D, Niyogi S, Bal M. A new method of plasmid DNA preparation by sucrose-mediated detergent lysis from Escherichia coli (gram-negative) and Staphylococcus aureus (gram-positive) Anal Biochem. 1989 Feb 1;176(2):344-9. Chen Y, Gin JW, Wang Y, de Raad M, Tan S, Hillson NJ, Northen TR, Adams PD, Petzold CJ. Alkaline-SDS cell lysis of microbes with acetone protein precipitation for proteomic sample preparation in 96-well plate format PLoS One. 2023 Jul 7;18(7):e0288102. Budel JCC, Hess MK, Bilton TP, Henry H, Dodds KG, Janssen PH, McEwan JC, Rowe SJ. Low-cost sample preservation methods for high-throughput processing of rumen microbiomes Anim Microbiome. 2022 Jun 6;4(1):39. Pinto FL, Thapper A, Sontheim W, Lindblad P. Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria BMC Mol Biol. 2009 Aug 7;10:79. Zierdt CH, Kagan RL, MacLowry JD. Development of a lysis-filtration blood culture technique J Clin Microbiol. 1977 Jan;5(1):46-50. Santiso R, Tamayo M, Gosálvez J, Bou G, Fernández Mdel C, Fernández JL. A rapid in situ procedure for determination of bacterial susceptibility or resistance to antibiotics that inhibit peptidoglycan biosynthesis BMC Microbiol. 2011 Aug 25;11:191. Mendonca AF, Amoroso TL, Knabel SJ. Destruction of gram-negative food-borne pathogens by high pH involves disruption of the cytoplasmic membrane Appl Environ Microbiol. 1994 Nov;60(11):4009-14.
