Terrific Broth Application: Nutritionally rich liquid medium designed for the growth and maintenance of recombinant Escherichia coli strains in molecular biology, supporting high cell density and extended logarithmic phase cultivation. Product Details: Dehydrated powder Appearance: Beige powder; amber-colored when prepared and autoclaved Storage: 2ºC to 25ºC; keep container tightly closed Preparation: Dissolve 47.6 g powder in 900 mL distilled water, add 4 mL glycerol, adjust volume to 1 L, heat to dissolve, autoclave at 121ºC for 15–20 min, store at 2ºC to 8ºC Formulation (g/L): Tryptone 12.00, Yeast Extract 24.00, K2HPO4 12.54, KH2PO4 2.31 Final pH: 7.2 ± 0.2 at 25ºC Key Features: Supports robust high-density growth of recombinant E. coli Phosphate buffer prevents acidification and supports cell viability Glycerol as carbon source avoids acetic acid buildup and glucose repression Rich mix of nutrients including carbon, nitrogen, vitamins, and minerals Compatible with addition of heat-sensitive additives post-sterilization Applications: Plasmid DNA production at high yield Recombinant protein expression studies Prolonged logarithmic phase cultivation Molecular and biotechnological research
