Bovine H-IVM Medium For the in vitro maturation of oocytes outside the incubator Description and Prod. No. 20 ml HEPES IVM medium in glass bottle, Prod. No. 1.04.020 The medium is HEPES buffered and does not require CO2 equilibration, but it can also be used in a CO2 Incubator (recommended without vial lid, if oocytes arrive to the laboratory before 20 hours of after IVM) The medium is ready-to-use and does not need any supplements Medium should be preheated to 38.8°C prior to use Key Ingredients: HEPES, FSH and LH, Epidermal Growth Factor (EGF), Midkine (MK), L-Carnitine, Sodium Pyruvate, Albumin, Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin Quality Control Tests and Manufacturing Sterility tested (Fungal and Bacterial) Osmolality tested and pH tested Endotoxin tested Manufacturer is ISO9001 and ISO13485 certified Controlled environment and to human standards Bovine Embryo Assay (BEA) Batch analysis certificate available Storage: Store in original unopened container refrigerated 3-9 °C and protected from light Do not expose to temperatures higher than 39°C Do not freeze product it may precipitate < 2°C The medium must be used within 10 days after opening, providing it has been handled aseptically and is kept refrigerated after opening Discard unused medium if it has been warmed
