Defined Bioscience’s HiDef® B8 is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state. HiDef® B8 is provided in two formats to offer flexibility for use: The HiDef® B8 complete medium kit, including the 400X Supplement vial and our DMEM/F12 basal medium; The HiDef® B8 400X Supplement only (basal medium NOT included), for the modern convenience of basal medium choice. To better support small-scale experiments and pilot studies, the HiDef-B8 400x Supplement is offered in two vial sizes. Standard vials prepare 500 mL of complete medium, while miniature vials prepare 50 mL, enabling convenient preparation of smaller volumes without excess waste. See instructions below regarding preparation of each format. More Details The HiDef® B8 medium is a unique formulation that offers the modern conveniences of a flexible feeding schedule (including weekend-free maintenance) and the ability to choose the matrices and passaging methods that best suit specific applications. HiDef® B8 contains insulin, ascorbic acid-2-phosphate, transferrin, sodium selenite, TGFB3, NRG1, and thermostable FGF2/bFGF. The addition of further components is not required. Defined Bioscience recommends the use of our DMEM/F12 (catalog # LSB-101) when preparing HiDef® B8 complete medium, as its composition is optimized specifically for HiDef-B8 use. HiDef® B8 400X Supplement compatibility has also been verified with several manufacturers’ formulations of DMEM/F12 such as Corning® (#10-092), Gibco™ (#11330), and GenClone™ (#25-503), maintaining human pluripotent stem cells on matrices from several manufacturers including Matrigel® (Corning® #356231), Geltrex™ (Gibco™ #A1569601), and ACS-3035™ Cell Basement Membrane (ATCC®) at coating concentrations as low as 2.4 µg cm-2 (e.g. ~1:800 dilution of 18 mg/mL stock matrix at 1 mL per well in 6-well TC- treated plates), in addition to defined substrates such as vitronectin (VTN-N, Gibco™ #A14700). This list of complementary components routinely used in PSC culture is for reference and is not an exhaustive list of verified product compatibility. Contact us at [email protected] for more information. Each lot of HiDef-B8 400X Supplement is used in combination with basal medium in performance testing in a culture assay using human iPSCs.
