Isolation / extraction of total RNA from a large number of mammalian tissues and cells and yeast. Provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. No need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated Applications: RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation High quality - high purity total RNA can be directly used in various sensitive downstream applications Fast - several samples can be extracted in 20 minutes by column method Safe - no phenol chloroform extraction required Sensitive - RNA can be recovered at the level of picogram Storage/Stability: store kit dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. See protocol details in manual: Kit Contents Product R401102 R401103 Preparation Times 50 250 HiPure RNA Mini Columns 50 250 2 mL Collection Tubes 50 250 RTL Lysis Buffer 40 mL 200 mL RNA Binding Buffer 15 mL 75 mL Buffer RW1 40 mL 200 mL Buffer RW2* 20 mL 2 x 50 mL RNase Free Water 6 mL 30 mL Citations: Louis L, Ramzan F, Martin DD, Olaitan AO.0.Reversion of heme-dependent metronidazole resistance in Clostridioides difficile. Microbiol Spectr0:e01787-25.https://doi.org/10.1128/spectrum.01787-25
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