Product Specifications Item# 1031: Recombinant HIV-1 YU2 (M Tropic)Envelope Glycoprotein gp120 ( Baculovirus) Concentration: See vial Diluent: PBS Purity: >95% Stabilizer: None Preservative: None Storage: -75°C Physical State: Frozen Liquid Stability: At least 6 months at -75°C. Application: In-Vitro Diagnostics, Cell Activation, CD4 Binding Description: Gene cloned from Macrophage -cell-tropic HIV-1 YU2 strain and the full length recombinant envelope gp120 glycoprotein produced in the Baculovirus Expression System. Purification: This protein is purified by immuno-affinity chromatography to >95% purity as determined by SDS-PAGE, reduced. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA. Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti gp120 mAb-peroxidase capture ELISA. Cell activation assays in progress. Application and Instructions for use Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions. Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 receptor binding and co-receptor binding studies.
