Human Adiponectin ELISA Kit

Biofargo Verified independent store

→

Background Adiponectin, also referred to as adipocyte complement-related protein of 30 kDa (Acrp30), is a protein secreted predominantly by adipocytes and exerts broad paracrine an d endocrine effects on metabolic homeostasis and inflammation. It plays a critical role in promoting adipocyte differentiation, enhancing fatty acid oxidation, and improving insulin sensitivity. Circulating adiponectin levels are inversely associated with obesity, type 2 diabetes mellitus, and atherogenesis, highlighting its potential protective role in metabolic and cardiovascular disorders. Structurally, adiponectin oligomerizes into ~70 kDa noncovalently linked trimers, which are stabilized through disulfide bonds to form medium-molecular-weight hexamers and subsequently assemble into high-molecular-weight oligomers exceeding 300 kDa, the latter being considered the most biologically active isoforms. Features Species: Human Size: 96 T Sample Volume: 50 μl / 10 μl Standard Curve Range: 6.86–5000 pg/ml Assay Type: Fully Ready-to-Use Sensitivity: 0.15 pg/ml (50 μl); 3.56 pg/ml (10 μl) Operation Duration: 120 min Assay Type: Sandwich ELISA Typical data pg/ml O.D. Average Corrected 0.00 0.0097 0.0102 0.0100 6.86 0.0277 0.0283 0.0280 0.0181 20.58 0.0665 0.0592 0.0629 0.0529 61.73 0.1897 0.1883 0.1890 0.1791 185.19 0.5615 0.5451 0.5533 0.5434 555.56 1.4160 1.4190 1.4175 1.4076 1666.67 2.8940 2.7930 2.8435 2.8336 5000.00 4.1026 4.1065 4.1046 4.0946 Precision Intra-assay Precision Inter-assay Precision Sample Number S1 S2 S3 S1 S2 S3 22 22 22 6 6 6 Average(pg/ml) 103.3 485.3 1632.6 111.5 520.8 1724.6 Standard Deviation 2.8 27.8 81.6 5.8 31.7 128.9 Coefficient of Variation(%) 2.7 5.7 5.0 5.2 6.1 7.5 Intra-assay Precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays): Three samples of known concentration were tested six times on different plates to assess inter-assay precision. Spike Recovery The spike recovery was evaluated by spiking three levels of human adiponectin into healthy human serum samples. The unspiked serum was used as a blank in this experiment. The recovery ranged from 81% to 126%, with an overall mean recovery of 93%. Sample values Sample Matrix Sample Evaluated Range (ng/ml) Detectable % Mean of Detectable (ng/ml) Serum 30 2621.9-7072.8 100 4559.1 Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human adiponectin in this assay. No medical histories were available for the donors. Storage Store at +4 °C. Please refer to the protocols. Research Use Only For research use only. Not for use in diagnostic procedures. Documents Human Adiponectin ELISA Kit Show more