Scientific backgroundC4d (Complement Component 4d) is a biologically relevant protein marker measured to support mechanistic studies and biomarker discovery (context dependent).Protein concentrations can change due to secretion, degradation, cell composition shifts, or post-transcriptional regulation, so ELISA readouts often add information beyond gene expression alone.Quantitative measurements help compare groups and time points using standardized curves and can be interpreted alongside phenotype and pathway-specific readouts.Why it matters Quantify C4d (Complement Component 4d) to compare biological changes across conditions, doses, or time points. Generate concentration data from a standard curve to support biomarker and mechanistic studies. How the ELISA worksDesigned for Human samples, this kit uses a The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4d. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4d. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4d, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C4d in the samples is then determined by comparing the OD of the samples to the standard curve.. After binding and washing, signal is converted to concentration using a standard curve.Sample types: serum, plasma and other biological fluids. Detection range: 2.19-140 ng/mL Sensitivity/LoD: 0.96 ng/mL Assay time: 3.5h
