Description SAMPLE TYPE Serum, plasma, cell culture supernatant, and other biological samples SAMPLE VOLUME 100 μL SENSITIVITY 0.18 ng/mL RANGE 3.13 ng/mL – 200 ng/mL ASSAY TIME 3.5 h RECOVERY 93% – 100% AVERAGE RECOVERY 1.04 INTRA PRECISION 5.5% – 7.6% INTER-PRECISION 5.6% – 8.1% PLATFORM ELISA PLATE Detachable 96-well plate SIZE 96T/48T STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. DELIVERY 4℃ blue ice transportation COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Human IgG monoclonal antibodyHuman IgG freeze-dried standardIgG detect AntibodyHRP-labeled streptavidinAssay Buffer(10×)Substrate TMBStop SolutionWashing Buffer(20×)Sealing Film ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IgG antibodies are precoated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, IgG present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, detection antibodies labeled with horseradish peroxidase (HRP) are added and incubated. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IgG in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). Citations A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide. Frontiers in Immunology 2022-10-19 Reduced Energy Metabolism Impairs T Cell-Dependent B Cell Responses in Patients With Advanced HBV-Related Cirrhosis Frontiers in Immunology 2021-06-23 Development of a mammalian cell-based ZZ display system for IgG quantification BMC BIOTECHNOLOGY 2023-07-28 Flower-like WSe2 used as bio-matrix in ultrasensitive label-free electrochemical immunosensor for human immunoglobulin G determination ANALYTICAL SCIENCES 2023-05-25 Documents Datasheet SDS File Documents Human IgG ELISA Kit - Multi Sciences User Manual
