Product Specifications: Item # 2503: Human Anti-p24 HIV-1 IIIB pAb IgG Concentration: See vial Mass/vial: 100ug Diluent: PBS Purity: >90% Stabilizer: None Preservative: None Storage: -75°C Physical State: Frozen Liquid Stability: At least 6 months at -75°C Minimum Dilution: 1 : 1000 Application: ELISA/p24 Western ELISA, Immunoprecipitation, Immunohistochemistry, Virus Neutralization Assays, FACS, p24 Capture ELISA. Description: pAb IgG fraction purified from HIV-1 immune human serum . Purification: This pAb IgG was purified by rp24 affinity chromatography to >95% purity as determined by SDS-PAGE, reduced. Specificity: This pAb IgG binds to native and recombinant HIV-1 p24 in ELISA and Western ELISA. Biological Activity: None Application and Instructions for use: Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized p24 (IDI Product# 1003) under standard conditions. ELISA and Western ELISA may be performed at 1-2µg/ml of pAb depending on the nature and the quantity of the immobilized antigen. Histochemical studies require dilutions in 1 : 200 dilution range. Caution: Avoid the use of needles and ensure proper laboratory safety precautions are in place for the use of this product.
