カテゴリ: 論文誌(論文単位)グループ名: 【A】基礎・材料・共通部門発行日: 2016/02/01タイトル(英語): Visualization of Spatially Distributed Bioactive Molecules using Enzyme-Linked Photo Assay著者名: Hikaru Mabuchi (Toyohashi University of Technology), HongYao Ong (Toyohashi University of Technology), Kazunori Watanabe (Toyohashi University of Technology), Sachiko Yoshida (Toyohashi University of Technology), Naohiro Hozumi (Toyohashi University of Te著者名(英語): Hikaru Mabuchi (Toyohashi University of Technology), HongYao Ong (Toyohashi University of Technology), Kazunori Watanabe (Toyohashi University of Technology), Sachiko Yoshida (Toyohashi University of Technology), Naohiro Hozumi (Toyohashi University of Technology)キーワード: bioactive molecules，enzyme-linked photo assay，independent component analysis要約(英語): In this paper, we propose a new simple device for visualizing bioactive molecules with a fine spatial resolution by using a membrane in which a specific enzyme is immobilized. The layer produces fluorescence after association with a specific substance. The layer, on which a biological tissue is to be mounted, is deposited on a quartz substrate that is used as a light guide to introduce UV light to the layer. Substance release is observed by a CCD camera from the opposite side of the substrate. In order to shorten the experiment time, we had automated the optical device. The paper also describes the reduction of background fluorescence by means of image processing technique. Images were acquired by employing two UV-LEDs with slightly different angle. Image processing was performed to separate background and target fluorescence by means of independent component analysis. Finally the release of GABA(γ-aminobutyric acid) and glutamate from specific layers in rat cerebellum was successfully observed. It is expected that, using this method, both real-time transmitter release and its response to medicine can be observed.本誌: 電気学会論文誌A（基礎・材料・共通部門誌） Vol.136 No.2 （2016） 特集：2014 International Symposium on Electrical Insulating Materials本誌掲載ページ: 99-104 p原稿種別: 論文／英語電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejfms/136/2/136_99/_article/-char/ja/
