カテゴリ: 論文誌(論文単位)グループ名: 【E】センサ・マイクロマシン部門発行日: 2014/03/01タイトル(英語): Fabrication of a Perfusable Glass Microfluidic Channel for Microtubule Manipulation using an Electric Field著者名: Tasuku Nakahara (Department of Micro Engineering, Kyoto University), Naoto Isozaki (Department of Micro Engineering, Kyoto University), Suguru Ando (Department of Micro Engineering, Kyoto University), Nagendra Kumar Kamisetty (Department of Micro Engineer著者名(英語): Tasuku Nakahara (Department of Micro Engineering, Kyoto University), Naoto Isozaki (Department of Micro Engineering, Kyoto University), Suguru Ando (Department of Micro Engineering, Kyoto University), Nagendra Kumar Kamisetty (Department of Micro Engineering, Kyoto University), Hirofumi Shintaku (Department of Micro Engineering, Kyoto University), Hidetoshi Kotera (Department of Micro Engineering, Kyoto University), Ryuji Yokokawa (Department of Micro Engineering, Kyoto University/Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST))キーワード: μTAS，Motor proteins，Molecular transport，Kinesin要約(英語): In this report, we propose a microfluidic device made of fused silica substrates and an optimized assay procedure for the device to reconstruct a motor protein system. The fabricated device is created using photolithography methods and bonding techniques to realize a minimum channel size of 4 μm in width and 2 μm in depth. The glass bonding process achieves good reproducibility by optimizing substrate cleaning processes, coating conditions, and the concentration of the sodium silicate solution. The assay sequence required to realize microtubule gliding in the channel without losing protein functionality is optimized to allow manipulation of the microtubule by applying an electric field. The average curvature of the microtubule trajectory is measured at 5.7±2.8 μm (N=3) at an electric field of 5 kV/m. The proposed device might have applications in micro total analysis systems.本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.134 No.3 （2014） 特集：センサ・マイクロマシン英文特集号本誌掲載ページ: 64-69 p原稿種別: 論文／英語電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/134/3/134_64/_article/-char/ja/
