Real-time High-resolution Measurement of Pancreatic β Cell Electrophysiology Based on Transparent Thin-film Transistor Microelectrode Arrays

電気学会 電子図書館 Verified independent store

→

カテゴリ: 論文誌(論文単位)グループ名: 【E】センサ・マイクロマシン部門発行日: 2022/10/01タイトル(英語): Real-time High-resolution Measurement of Pancreatic β Cell Electrophysiology Based on Transparent Thin-film Transisto r Microelectrode Arrays著者名: Dongchen Zhu (Chemical System Engineering, The University of Tokyo), Anne-Claire Eiler (Institute of Industrial Science, The University of Tokyo), Satoshi Ihida (Institute of Industrial Science, The University of Tokyo), Yasuyuki Sakai (Chemical System En著者名(英語): Dongchen Zhu (Chemical System Engineering, The University of Tokyo), Anne-Claire Eiler (Institute of Industrial Science, The University of Tokyo), Satoshi Ihida (Institute of Industrial Science, The University of Tokyo), Yasuyuki Sakai (Chemical System Engineering, The University of Tokyo), Hiroshi Toshiyoshi (Institute of Industrial Science, The University of Tokyo), Agnes Tixier-Mita (Institute of Industrial Science, The University of Tokyo), Kikuo Komori (Chemical System Engineering, The University of Tokyo/Department of Biotechnology and Chemistry, Kindai University)キーワード: thin film transistor，microelectrode array，pancreatic β cell要約(英語): A transparent high density thin-film-transistor microelectrode array (TFT-μEA) was investigated, for the first time, to apply to real-time electrophysiological monitoring on glucose-stimulated insulin secretion dynamics of pancreatic β cells at higher resolution than conventional microelectrode arrays (MEAs). TFT-μEAs employed in this work are designed based on the switch matrix architecture, which incorporates a large sensing area (15.6 mm × 15.6 mm) with a 150 × 150 array of indium-tin-oxide (ITO) microelectrodes placed at a 100 μm pixel pitch. TFT-μEAs coated with poly-L-lysine and laminin enabled to culture rat insulinoma β line iGL for at least 7 days without cell death, which was determined by conventional cell viability tests based on a fluorescent staining method. Real-time action potentials of iGL cells stimulated by 15 mM glucose were successfully observed in similar to those in a conventional MEAs. These results are the first step towards the development of a multimodal TFT-μEAs device for electrophysiological, biochemical and optical analyses of the pancreatic islets. TFT-μEAs would extremely be promising platforms in the bioanalysis field for neurochemistry and electrophysiology.本誌: 電気学会論文誌E（センサ・マイクロマシン部門誌） Vol.142 No.10 （2022）本誌掲載ページ: 266-272 p原稿種別: 論文／英語電子版へのリンク: https://www.jstage.jst.go.jp/article/ieejsmas/142/10/142_266/_article/-char/ja/ Show more