The PELCO ColdSpot® Pro 36116-10 designed as a temperature controlled surfacewill enhance most tissue processing steps The PELCO ColdSpot® Pro is designed for control of the environment inside the PELCO BioWave® Pro and Pro+ microwave systems. The water within the PECLO ColdSpot® Pro circulates through the internal Load Cooler within the BioWave® system or through the external PELCO SteadyTemp™ Pro chiller. This provides consistent temperature control at the specimen level as well as eliminating the standing wave pattern within the microwave cavity. The PELCO ColdSpot® Pro is designed for microwave processing techniques that benefit from an efficient heat transfer between the PELCO ColdSpot® surface and the sample. This product design provides excellent temperature control for electron microscopy tissue processing, immunolabeling and in situ hybridization protocols. The glass surface dissipates heat quickly and efficiently. This design creates a flat surface across the entire glass top enabling a wide range of usage with consistency. The temperature of the water inside this PELCO ColdSpot® should not exceed 60°C (140° F) because the thermal properties of the plastic and the adhesive will be compromised at higher temperatures. All PELCO® Microwave processors can be set to maintain the temperature below this critical point. Designed for use with 3536 PELCO® Vacuum Chamber. Glass plate area dimensions: 223 W x 291mm D (8.78 W x 11.34" D)Overall dimensions: 267 W x 381 D x 108mm H (10.5 W x 15 D x 4.25" H) PELCO® EM Pro Microwave Vacuum Chamber 3536 The PELCO® EM Pro Vacuum Chamber is made from microwave-transparent polypropylene and designed to reach a maximum vacuum of 20" of Hg. The PELCO® EM Pro Vacuum Chamber works in conjunction with the internal vacuum pump in the PELCO BioWave® Pro and Pro+ microwave systems. The vacuum chamber can run a continuous vacuum or utilize a vacuum cycle, depending on the application and need of the user. Features Made of chemically resistant, microwave-transparent polypropylene and designed for vacuum down to 20" of Hg (1 torr/1mm Hg) and temperatures up to 70°C. Designed either to run a continuous vacuum during microwave processing or a vacuum cycle. Improves fixative penetration and ultrastructural preservation during microwave-assisted chemical fixation. Improves resin infiltration in the microwave and greatly reduces the turnaround times when compared to established microwave protocols. Can be used in or outside of the PELCO BioWave® Pro Microwave Tissue Processor. Dimensions Inside Chamber 178mm diameter x 84mm height(6.663" dia. x 3.313" height) Overall Dimensions 203mm diameter x 111mm height (8.000" dia. x 4.373" height) 3536-10 Top Plate with Temperature Probe Port (optional). Note that the Temperature Probe with cable connection for the PELCO BioWave® Pro Microwave Tissue Processor is inserted (also optional)See below for all accessories. PELCO® EM Pro Vacuum Chamber shown with optional Top Plate with temperature probe port and optional Temperature Probe in place.See Table 1 The effect of Microwaves/Vacuum on the Sample Processing Times for Electron Microscopy Table 1: The Effect of Microwaves/Vacuum on the Sample Processing Times for Electron Microscopy Processing Step Routine Microwave Processing 1 Vacuum MicrowaveProcessing 2 Routine Processing 1. Primary Fixation (aldehyde) .....10 minutes .....6 minutes .....1 hour 2. Buffer Rinse .....6 minutes .....4 minutes .....0.5 hours 3. Secondary Fixation (osmium) .....10 minutes .....6 minutes .....1 hour 4. Dehydration (acetone/ethanol) .....7 minutes .....7 minutes .....2 hours 5. Resin Infiltration .....50 minutes .....8 minutes .....18 hours 6. Tissue to Embedding Capsules .....15 minutes .....15 minutes .....0.25 hours 7. Resin Polymerization .....45-75 minutes .....45-75 minutes .....18 hours TOTALS .....~170 minutes .....~120 minutes .....~2,400 minutes Note: we recommend using the new PELCO BioWave® Pro+ Microwave Tissue Processor with built-in manual or programmable Vacuum Pump.If not available, we recommend the 3436 Diaphragm Vacuum Pump to provide vacuum to this vacuum chamber. 1/4" ID Silicone Vacuum Hose 92490-253, 92490-256 Thick walled silicone vacuum hose with 1/4" (6.35mm) inside diameter and 1/2" (12.7mm) outside diameter. Sturdy, yet flexible material with wall thickness of 1/8" (3.2mm), will not collapse under vacuum. Sold in lengths of 3 and 9 ft. Squenza Slide Rack and Coverplate System The Sequenza™ Slide Rack, holding 10 glass slides and Coverplates™, is a system designed for the immunolabeling of sections on glass slides. While originally designed for bench techniques, this system is uniquely suited for microwave-assisted applications. The Slide Rack holds glass slides with a Coverplate™. This combination forms a capillary gap between the slide and the plate. Reagent volumes, especially antibody, are greatly reduced using this versatile system. A reagent hopper forms the uppermost part of the plastic Coverplate™ allowing for easy dispensing of reagent into the capillary gap. Using a PELCO® Microwave Processor with a PELCO ColdSpot®, rapid immunostaining of sections on glass slides is reliable and easy. 36105 & 36107 Clean with 10% bleach solution and rinse with well water. Sequenza™ Slide Rack and Coverplate™ system is not dishwasher safe. PELCO® Technical Notes, Coverplate™ Technology (127KB PDF) PELCO Prep-Eze™ Wellplate Inserts The rectangular polypropylene Wellplate Inserts have 6, 12, or 24 wells and will fit into the Corning Costar® Tissue Culture Plates of 6/12/24 Wellplate design. A 420µm opening, polypropylene mesh is at the bottom of each of the insert wells. The Wellplate Inserts may only be chemically sterilized. The outside dimensions of the Wellplate Inserts are: 81.3mm W x 123.5mm L x 24mm H (3.2" W x 4.86" L x 0.88" H) Well dim. of 6-wellplate inserts: 24.1mm dia x 22.3mm D (0.95" dia x 0.88" D)Well dim. of 12-wellplate inserts: 14.2mm dia x 22.3mm D (0.56" dia x 0.88" D)Well dim. of 24-wellplate inserts: 11.2mm dia x 22.3mm D (0.44" dia x 0.88" D)Depth of well from top to bottom on all Wellplate Inserts: 17.68mm (0.696") 36168PELCO Prep-Eze™6-Wellplate Insert 36170PELCO Prep-Eze™12-Wellplate Insert 36172PELCO Prep-Eze™24-Wellplate Insert PELCO® Technical Notes, PELCO Prep-Eze™ Rectangular Wellplate Inserts - Instructions (346KB PDF)
