Description Our bioactive Interleukin-2 (IL-2) Human, E. coli Recombinant Protein is a single, non-glycosylated polypeptide chain containing 133 amino acids and has a molecular mass of 15.4 kDa. The IL-2 is purified by proprietary chromatographic techniques. Summary IL-2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. Other Names Interleukin 2, IL2, T-cell growth factor (TCGF), UniProtKB# P60568 Amino Acid Sequence APTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLT. Source E. coli Formulation Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a 0.2 μm filtered concentrated solution (1.0 mg/ml) in PBS, pH 5.0. Purity Greater than 97.0% as determined by: Analysis by RP-HPLC. Analysis by SDS-PAGE. Biological Activity The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 1x107 IU/mg. Reconstitution It is recommended to reconstitute the lyophilized Interleukin-2 in sterile distilled water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Shipping At ambient temperature. Upon receipt, store the product at the temperature recommended below. Storage/Expiration Lyophilized IL-2, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, IL-2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Usage This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive. Resources Product Datasheet (PDS) Related Products Cytokines & Growth Factors ISOkine® IL-2 Human, Animal-Free Recombinant Protein IL-2 Mouse, E. coli Recombinant Protein
