Description L-Asparaginase enzyme purified from E. coli ASI.357 is a tetrameric polypeptide chain with a molecular mass of 34,564 Dalton. Summary L-Asparaginase is an enzyme that depletes L-Asparagine, an important nutrient for cancer cells, resulting in cancer/tumor cell starvation. L-asparaginase is an anti-tumor agent derived from E. coli which can inhibit the growth of malignant cells. It is used mainly for the induction of remission in acute lymphoblastic leukemia. Because of the lymph node origin of malignant B cells in Multiple Myeloma, L-Asparagine is an essential amino acid for their cell metabolism, and, consequently, L-Asparaginase may be of value in managing the disease. The rationale behind asparaginase is that it takes advantage of the fact that acute lymphoblastic leukemia cells are unable to synthesize the non-essential amino acid asparagine whereas normal cells are able to make their own asparagine. These leukemic cells depend on circulating asparagine. Asparaginase catalyzes the conversion of L-asparagine to aspartic acid and ammonia. This deprives the leukemic cell of circulating asparagine. Source L-Asparaginase was purified from E. coli ASI.357 CAS Registry No. 9015-68-3 Formulation The L-Asparaginase enzyme was lyophilized with no additives. Purity Greater than 96.0% as determined by SDS-PAGE. Reconstitution It is recommended to reconstitute the lyophilized L-Asparaginase in 18MOhm water at 1 mg/ml. Unit Definition One unit of enzyme catalyzes hydrolyzation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Biological Activity One IU of L- Asparaginase is defined as that amount of enzyme required to generate 1 µmol of ammonia per minute at pH 7.3 and 37°C. Shipping Ships at ambient temperature. Upon receipt, store the product at the temperature recommended below. Storage/Expiration Lyophilized L-Asparaginase, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution L-Asparaginase should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. Usage This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive. Other Names L-ASNase, L-asparagine amidohydrolase, ansA Resources Product Datasheet (PDS)
