Lab-Aids: Basic Chromatography Kit This kit is an exceptional introduction to paper chromatography. Students learn how chromatography can be used to identify the makeup of organic and inorganic substances. To accomplish this, they perform two basic experiments:‚ The separation of chlorophyll extract into its constituent pigments‚ The separation of dyes with polar and non-polar solvents demonstrating how chromatography is used to determine the makeup of various organic and inorganic substances. Scientific Concepts: Understand how chromatography can be used to identify the makeup of organic and inorganic substances Perform chromatography to identify the constituent pigments of chlorophyll Observe differences in polar and non-polar solvents with dyes Content List: 1 Teacher's Guide with MSDS 30 Chromatography tubes and holders 120 Chromatography strips, Pre-cut paper 1 Developer I (petroleum ether and acetone), Bottle 1 Developer II (deionized water), Bottle 1 Developer III (n-butanol), Bottle 1 Developer IV (n-butanol, acetic acid and deionized water), Bottle 1 Capillary applicators, Vial 1 Chloroplast Extract, concentrated, Vial 1 Dyes, mixed, Vial Classroom Planning: To complete this kit requires 1 ~50-minute class period. Number of Students: 30 (if working individually, more if grouped)
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